Fluidic separation and detection

Patent metadata

Field	Value
Publication number	US-2016266138-A1
Application number	US-201415036455-A
Country	US
Kind code	A1
Filing date	Nov 14, 2014
Priority date	Nov 14, 2013
Publication date	Sep 15, 2016
Grant date	—

Abstract

A method for analyzing a component using a fluidic device. The method includes the steps of providing a distribution of the component across contacting first and second fluid flows; diverting a part of the first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, wherein the diverted part includes the component; and analyzing the component in the diverted part of the fluid flow. Optionally the component may be labelled prior to the analyzing step. A flow apparatus for use in the method is also provided.

First claim

1 . A method for analysing analyzing a component, the method comprising the steps of:
(iii) providing a distribution of the component across contacting first and second fluid flows, such as laminar fluid flows; (iv) diverting a part of the first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, wherein the diverted part comprises the component; (v) subsequently labelling the component in the diverted part of the fluid flow; and (vi) analyzing the component in the diverted part of the fluid flow.

2 . The method of claim 1 , the method comprising the preliminary steps of:
(i) providing the component in a first fluid flow; (ii) contacting the fluid flow with a second fluid flow, such as to generate a laminar flow; and step (iii) is permitting the component to join the second fluid flow, thereby to obtain a distribution of the component across the first and second fluid flows.

3 . The method of claim 1 , wherein step (v) is quantitative labelling.

4 . The method of claim 1 , wherein the lateral distribution of the component across the first and second fluid flows is non uniform.

5 . The method of claim 1 , wherein step (iii) comprises the diffusion of the component into the second fluid flow.

6 . The method of claim 1 , wherein step (iii) comprises the electrophoretic movement of the component into the second fluid flow.

7 . The method of claim 1 , wherein step (iv) is diverting a part of the second fluid flow, wherein the diverted part comprises the component.

8 . The method of claim 1 , wherein the label is a latent label, such as OPA.

9 . The method of claim 1 , wherein step (v) is fluorescent labelling of the component.

10 . The method of claim 1 , wherein the component is or comprises a protein, a polypeptide, a polynucleotide or a polysaccharide.

11 . (canceled)

12 . (canceled)

13 . The method of claim 1 , wherein the component is a component of a multicomponent mixture.

14 . The method according to claim 13 , wherein the component is monomeric or is an aggregate, and other components in the multicomponent mixture are selected from monomers and aggregates, wherein the other components are not the same as the component.

15 . The method of claim 2 , wherein the component joins the second fluid flow in step (ii) in a native state.

16 . The method of claim 15 , wherein the component joins the second flow in step (iii) in a native state.

17 . The method of claim 1 , wherein the component is analysedanalvzed in step (vi) in a denatured state.

18 . The method of claim 1 wherein step (vi) includes determining the dry mass of the component in the diverted flow.

19 . A flow apparatus for detecting a component in a mixture, the apparatus comprising a separation channel for first and second flows in contact, and the separation channel is in fluid communication with a downstream flow separator, and a detection zone which is downstream and in fluid communication with the flow separator, wherein the separation channel is adapted to permit lateral movement of components between contacting first and second flows and the flow separator is adapted to divert a part of the first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, from the separation channel.

20 . The flow apparatus of claim 19 , wherein the detection zone is provided with a detection channel that is downstream of the flow separator and is for receiving diverted fluid from the flow separator, and the detection zone is further provided with a denaturing channel for supplying denaturant to the detection channel at a first junction.

21 . The flow apparatus of claim 20 , wherein is further provided with a label channel for supplying label to the detection channel at a second junction, wherein the second junction is located downstream of the first junction.

22 . The flow apparatus of claim 19 , wherein the detection zone is provided with an detection channel that is downstream of the flow separator and is for receiving diverted fluid from the flow separator, and the detection zone is further provided with a label channel for supplying label to the detection channel at a second junction.

Assignees

• Cambridge Entpr Ltd

Inventors

• Yates Emma
• Dobson Christopher
• Herling Therese
• Knowles Tuomas

Classifications

• G01N2015/0038

  Investigating nanoparticles · CPC title

• B01L2300/0883

  Serpentine channels · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• B01L2200/0631

  Purification arrangements, e.g. solid phase extraction [SPE] · CPC title

• G01N27/447

  using electrophoresis · CPC title