System and Method for Determining Quality of Stem Cell Derived Cardiac Myocytes

Patent metadata

Field	Value
Publication number	US-2016203262-A1
Application number	US-201414913925-A
Country	US
Kind code	A1
Filing date	Aug 21, 2014
Priority date	Aug 23, 2013
Publication date	Jul 14, 2016
Grant date	—

Abstract

The present invention relates to a system and method for calculating a quality index of a differentiated cell. To calculate the quality index, the present invention measures a differentiated cell by at least one metric, calculates a strictly standardized mean difference between the differentiated cell and a targeted cell, and calculates a mean squared error versus the target cell to define a value that represents the total difference between the differentiated cell and targeted cell based on the at least one measured metric.

First claim

1 . A method for calculating a quality index of a differentiated cell, comprising:
measuring a differentiated cell by at least one metric; calculating a normalized residue between the differentiated cell and a targeted cell; and calculating a mean squared error (MSE) versus the target cell to define a value that represents the total difference between the differentiated cell and targeted cell based on the at least one measured metric.

2 . The method of claim 1 , wherein the at least one metric is selected from the group consisting of genetic information, electrophysiological information, structural information, and contractile information.

3 . The method of claim 2 , wherein the at least one metric comprises genetic information, electrophysiological information, structural information, and contractile information.

4 . The method of claim 1 , wherein the normalized residue is a strictly standardized mean difference (β).

5 . The method of claim 4 , wherein β is calculated according to the formula:

β
=

μ
1

-

μ
2

σ
1
2

+

σ
2
2

where μ represents mean and σ represents standard deviation.

6 . The method of claim 5 , wherein MSE is calculated according to the formula:

MSE
=

1
n



∑

i
=
1

n





β
i
2

7 . The method of claim 1 , wherein the differentiated cell is derived from a potent cell.

8 . The method of claim 7 , wherein the potent cell is a stem cell.

9 . The method of claim 8 , wherein the differentiated cell is a myocyte.

10 . The method of claim 9 , wherein the at least one metric is a sarcomere packing density.

11 . The method of claim 1 , wherein information pertaining to the targeted cell is a predetermined value related to the at least one metric.

12 . The method of claim 1 , wherein a lower MSE value is indicative of greater similarity between the differentiated cell and the targeted cell.

13 . A system for calculating a quality index of a differentiated cell, comprising a software platform run on a computing device that calculates a normalized residue between a differentiated cell and a targeted cell, and calculates a mean squared error (MSE) versus the target cell to define a value that represents the total difference between the differentiated cell and targeted cell based on at least one measured metric of the differentiated cell.

14 . The system of claim 13 , wherein the normalized residue is a strictly standardized mean difference (β).

15 . The system of claim 14 , wherein β is calculated according to the formula:

β
=

μ
1

-

μ
2

σ
1
2

+

σ
2
2

where μ represents mean and σ represents standard deviation.

16 . The system of claim 15 , wherein MSE is calculated according to the formula:

MSE
=

1
n



∑

i
=
1

n





β
i
2

17 . The system of claim 13 , wherein the at least one metric is selected from the group consisting of genetic information, electrophysiological information, structural information, and contractile information.

18 . The system of claim 17 , wherein the at least one metric comprises genetic information, electrophysiological information, structural information, and contractile information.

19 . The system of claim 13 , wherein a lower MSE value is indicative of greater similarity between the differentiated cell and the targeted cell.

20 . The system of claim 13 , wherein the differentiated cell is derived from a potent cell.

21 . The system of claim 19 , wherein the potent cell is a stem cell.

22 . The system of claim 13 , wherein the differentiated cell is a myocyte.

23 . The system of claim 21 , wherein the at least one metric is a sarcomere packing density.

Assignees

• Harvard College

Inventors

• Sheehy Sean Paul
• Pasqualini Francesco
• Parker Kevin Kit

Classifications

• G01N33/5091

  for testing the pathological state of an organism · CPC title

• G01N33/5005

  involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025) · CPC title

• G16B40/00

  ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding · CPC title

• G01N33/5073 Primary

  Stem cells · CPC title

• G06F19/24 Primary

  Physics · mapped topic