Electric field directed loading of microwell array

Patent metadata

Field	Value
Publication number	US-2016202209-A1
Application number	US-201615040815-A
Country	US
Kind code	A1
Filing date	Feb 10, 2016
Priority date	Oct 3, 2011
Publication date	Jul 14, 2016
Grant date	—

Abstract

An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. The apparatus further includes a wall structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors. The well structure including an electrode layer and an insulative layer.

First claim

What is claimed is:

1 . An apparatus comprising:
a device substrate including an array of sensors, each sensor of the array of sensors including an electrode structure disposed at a surface of the device substrate; and a well structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors, the well structure including an electrode layer and an insulative layer.

2 . The apparatus of claim 1 , wherein a well of the array of wells is to provide fluid access to a sensor of the array of sensors.

3 . The apparatus of claim 1 , wherein a well of the array of wells exposes the electrode structure of a sensor of the array of sensors.

4 . The apparatus of claim 1 , wherein the electrode layer is exposed in a plurality of wells of the array of wells.

5 . The apparatus of claim 1 , wherein the electrode layer is electrically connected to a plurality of wells of the array of wells.

6 . The apparatus of claim 1 , further comprising an electrical interconnect separate from the array of wells providing electrical access to the electrode layer.

7 . The apparatus of claim 1 , wherein the insulative layer is disposed below the electrode layer and further comprising a second insulative layer disposed above the electrode layer, the array of wells defined through the insulative layer, the electrode layer, and the second insulative layer.

8 . The apparatus of claim 1 , wherein a sensor of the sensor array is an ion sensitive field effect transistor.

9 . The apparatus of claim 1 , wherein the electrode structure includes a floating electrode.

10 . The apparatus of claim 1 , wherein the electrode structure is electrically isolated from the electrode layer except through fluid within the array of wells.

11 . The apparatus of claim 1 , further comprising a cover defining a flow cell over the wall structure, the flow cell to contain a fluid.

12 . The apparatus of claim 1 , further comprising a counter electrode and a voltage source electrically coupled to the electrode layer and the counter electrode.

13 . An apparatus comprising:
a device substrate including an array of sensors, each sensor of the array of sensors including an electrode structure disposed at a surface of the device substrate; and a well structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors, the well structure including a first insulative layer, an electrode layer disposed over the first insulative layer, and a second insulative layer disposed over the electrode layer.

14 . The apparatus of claim 13 , wherein a well of the array of wells exposes the electrode structure of a sensor of the array of sensors.

15 . The apparatus of claim 13 , wherein a sensor of the sensor array is an ion sensitive field effect transistor.

16 . The apparatus of claim 13 , wherein the electrode structure includes a floating electrode.

17 . The apparatus of claim 13 , wherein the electrode structure is electrically isolated from the electrode layer except through fluid within the array of wells.

18 . The apparatus of claim 13 , further comprising a counter electrode and a voltage source electrically coupled to the electrode layer and the counter electrode.

19 . An apparatus comprising:
a device substrate including an array of sensors, each sensor of the array of sensors including an electrode structure disposed at a surface of the device substrate; and a well structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors, a wall of a well of the array of wells exposed to a first insulative layer, an electrode layer disposed over the first insulative layer, and a second insulative layer disposed over the electrode layer, the well to provide fluid access to a sensor of the array of sensors, wherein the electrode structure is electrically isolated from the electrode layer except through fluid within the array of wells.

20 . The apparatus of claim 19 , further comprising a counter electrode and a voltage source electrically coupled to the electrode layer and the counter electrode.

Assignees

• Life Technologies Corp

Inventors

• Barbee Kristopher
• Davidson John F
• Hinz Wolfgang
• Li Shifeng
• Bustillo James

Classifications

• C12Q1/6874

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title

• B01J2219/00286

  Reactor vessels with top and bottom openings · CPC title

• B01J2219/00468

  by manipulation of individual beads · CPC title

• B01J2219/00596

  Solid-phase processes · CPC title

• B01J2219/00466

  in a slurry · CPC title