Capture system of cells and methods

Patent metadata

Field	Value
Publication number	US-2016178490-A1
Application number	US-201514976043-A
Country	US
Kind code	A1
Filing date	Dec 21, 2015
Priority date	Dec 22, 2014
Publication date	Jun 23, 2016
Grant date	—

Abstract

Embodiments of the disclosure include systems and methods for capturing particular biological matter from a sample from an individual. The systems and methods employ an apparatus (such as a container that is a tube or bag) comprising a fluoropolymer coating that is modified to comprise one or more moieties for binding of a desired biological agents when a sample is exposed to the modified fluoropolymer. In particular aspects, the biological matter is a particular type of cell, such as a particular blood or bone marrow cell, and the desired cell is captured by means of an aptamer selected to bind the cell.

First claim

What is claimed is:

1 . An isolation system comprising a container, wherein said container comprises:
an inner surface comprising a polymer having a total organic carbon (TOC) in water of less than 0.1 mg/cm 2 , and a plurality of functional groups attached to the polymer.

2 . The system of claim 1 , wherein the functional groups are directly or indirectly attached to a biological agent-capturing moiety.

3 . The system of claim 1 , wherein the functional groups are attached to the polymer through a linker.

4 . The system of claim 1 , wherein the functional groups have attached directly thereto a first member of a binding pair.

5 . The system of claim 4 , wherein the biological agent-capturing moiety comprises a second member of a binding pair.

6 . The system of claim 2 , wherein the functional groups have attached directly thereto a first member of a binding pair and a biological agent-capturing moiety comprises a second member of a binding pair, wherein said first and second members of the binding pair are bound to each other.

7 . The system of claim 2 , wherein the biological agent-capturing moiety binds a biological agent directly.

8 . The system of claim 2 , wherein the biological agent-capturing moiety indirectly binds a biological agent or produces a molecule that directly binds a biological agent.

9 . The system of claim 8 , wherein the biological agent-capturing moiety or the molecule produced by the biological agent-capturing moiety is nucleic acid.

10 . The system of claim 9 , wherein the biological agent-capturing moiety comprises a nucleic acid template.

11 . The system of claim 10 , wherein the nucleic acid template comprises sequence that is complementary to sequence that directly binds to the biological agent.

12 . The system of claim 8 , wherein the molecule produced by the biological agent-capturing moiety comprises one or more aptamers.

13 . A method of preparing the system of claim 1 , comprising the steps of:
providing a container comprising an inner surface comprising a polymer having a TOC in water of less than 0.1 mg/cm 2 ; and attaching functional groups to the inner surface.

14 . The method of claim 13 , further comprising the step of attaching a first member of a binding pair to the functional groups.

15 . The method of claim 14 , further comprising the step of attaching a second member of a binding pair to a biological agent-capturing moiety.

16 . The method of claim 15 , wherein the biological agent-capturing moiety is a circular DNA template and the method further comprises the step of providing a polymerase and nucleotides to the container.

17 . The method of claim 13 , wherein the attaching step is comprised of a plasma reaction followed by a wet chemical reaction.

18 . A method for isolating particular cells from a sample from at least one individual, comprising the steps of:
providing the system of claim 2 ; and subjecting to the system a sample comprising a mixture of cells under conditions that the particular cells in the mixture are able to selectively bind to the biological agent-capturing moiety or selectively bind to a molecule produced by the biological agent-capturing moiety.

19 . The method of claim 18 , wherein the subjecting step further comprises providing an enzyme to the system.

Assignees

• Saint Gobain Performance Plast

Inventors

• Civel Edouard
• Clark Sarah Louise
• Cullis Herbert Myers
• Lundgren Natasha Anna
• Miripol Jeffrey Ellis
• Garces Camila A

Classifications

• G01N1/405 Primary

  by adsorption or absorption · CPC title

• G01N33/54353

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• C12M47/04 Primary

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• C08F259/08

  on to polymers containing fluorine · CPC title

• G01N33/543

  with an insoluble carrier for immobilising immunochemicals · CPC title