Self-triggered flow cytometer

Patent metadata

Field	Value
Publication number	US-2016169809-A1
Application number	US-201514965141-A
Country	US
Kind code	A1
Filing date	Dec 10, 2015
Priority date	Dec 10, 2014
Publication date	Jun 16, 2016
Grant date	—

Abstract

An optical spectrum analyzer ( 100 ) having a an excitation light source ( 103 ) with a luminated bio-sample ( 114 ) carried by a flow path ( 102 ). A spectrum dispersive element 213 dispersants lumineses light generated by the bio-sample ( 114 ).

First claim

We claim:

1 . An optical spectrum analyzer comprising:
an excitation light source; an illuminated bio sample carried by a flow path, the bio-sample luminescences; a spectrum dispersive element, wherein at least a portion of the luminescence is directed to the spectrum dispersive element, wherein the luminescence is dispersed; a photo detection device having at least one photo detection input signal and at least one photo detection output, wherein a least a portion of the dispersed luminescence is inputted into the photo detection device; a digital signal processor, the storage space having a digital storage space input and a digital storage space output; an analysis space; and a digital trigger coupled to the photo detection output and when a signal is sensed wherein the, digital storage, and the digital processing unit stores and analyses data from the photo detection device.

2 . The optical spectrum analyzer as claimed in claim 1 wherein the excitation light source is a laser diode.

3 . The optical spectrum analyzer as claimed in claim 1 wherein the photo-detector unit (device) is an avalanche photo diode array (APD).

4 . The optical spectrum analyzer as claimed in claim 3 wherein the avalanche photo diode array (ADP) includes photo receiving elements ranging from six (6) to thirty-four (34).

5 . The optical spectrum analyzer as claimed in claim 1 wherein the digital processing unit starts to store data when triggered by received data from the array photo detection unit.

6 . The optical spectrum analyzer as claimed in claim 5 wherein all the data or a selected number of elements in the photo detection array is a logical add.

7 . The optical spectrum analyzer as claimed in claim 1 wherein the bio sample emits auto-luminescence.

8 . The optical spectrum analyzer as claimed in claim 1 wherein the bio-sample emits luminescence from marker on the bio-cell.

9 . The optical spectrum analyzer as claimed in claim 1 wherein the spectrum dispersive unit is a grating.

10 . The optical spectrum analyzer as claimed in claim 1 wherein the spectrum dispersive element is a prism.

11 . A bio-counting device comprising:
an excitation light source; an illuminated bio sample carried by a flow path; a spectrum dispersive element; a photo detection device having at least one photo detection input signal and at least one photo detection output; one photo detection output signal; a digital storage space having a digital storage space input and a digital storage space output; an analysis space; and a digital trigger coupled to the photo detection output and when a signal is sensed, wherein the digital storage space, and the digital processing unit stores and analyses data from the photo detection device.

12 . The bio-counting device as claimed in claim 11 wherein the excitation light source is a laser diode.

13 . The bio-counting device as claimed in claim 11 wherein the photo-detector is an avalanche photo diode array (APD).

14 . The bio-counting device as claimed in claim 13 wherein the avalanche photo diode array (APD) includes photo diode elements ranging from six (6) photo diodes to thirty four (34) photo diodes.

15 . The bio-counting device as claimed in claim 14 as claimed in claim 11 wherein the digital processing unit starts to store data when triggered by received data from the array photo detection unit

16 . The bio-counting device as claimed in 15 wherein all the data or a selected number of elements in the photo detection array is a logical add.

17 . The bio-counting device as claimed in 15 wherein the bio-sample emits auto-luminescence.

18 . The bio-counting device as claim 11 wherein the bio-sample emits luminescence from markers on the bio cell.

19 . The bio-counting device as claim 11 , wherein the spectrum dispersive element is a grating.

20 . A selective self-triggered flow-cytometer consisting of an excitation laser diode;
an luminating bio sample carried by a flow path and excited by the laser diode; a spectrum dispersive element dispersing luminescence from the illuminating bio sample; and an avalanche photodiode array detecting the luminance spectrum; and analysis the bio-cell using data from the avalanche photodiode array when triggered by the logic add of the correspondent number of elements of the photo diode array.

21 . A selective self-triggered flow-cytometer as claimed in claim 20 , wherein the avalanche photodiode array is made, in part, of photo receiving elements from eight (6) to elements to thirty (34).

Assignees

• Cytek Biosciences Inc

Inventors

• Jiang Wenbin

Classifications

• G01N2015/1486

  Counting the particles · CPC title

• G01N15/1436 Primary

  the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell · CPC title

• G01N2015/1006

  for cytology · CPC title

• G01N15/1459

  the analysis being performed on a sample stream · CPC title

• G01N15/1434

  Optical arrangements · CPC title