Decreasing bacterial responses on nano-modified titanium

Patent metadata

Field	Value
Publication number	US-2016166391-A1
Application number	US-201414570374-A
Country	US
Kind code	A1
Filing date	Dec 15, 2014
Priority date	Dec 15, 2014
Publication date	Jun 16, 2016
Grant date	—

Abstract

Methods of manufacturing produce metal implants having nano-modified surfaces that contain antimicrobial properties. The methods may include immersing the implant in an acid, rinsing the acid-treated implant in an aqueous cleaner, and thereafter heating the rinsed implant. The nano-modified implants described herein may contain an increased surface roughness; surface features with increased width or height; and/or decreased surface energy. The implants that result from these methods contain a nano-modified surface that is resistant to microbial cell adhesion and ultimately reduce biomaterials-related infections at the implant site.

First claim

1 . A method of manufacturing a implant, comprising:
a) immersing the implant in acid at a concentration of 0.5N to 15N for 5 minutes to 120 minutes; b) rinsing the acid-treated implant in an aqueous cleaner having a pH of 6 to 8 to remove the acid; and c) heating the rinsed implant at a temperature between 100° Celsius and 800° Celsius for a minimum of 30 minutes.

2 . The method of claim 1 , wherein the implant is a metal selected from the group consisting of commercially pure titanium, and titanium alloy.

3 . The method of claim 1 , wherein at least a portion of implant is porous prior to immersing in the acid.

4 . The method of claim 1 , wherein the implant is non-porous prior to immersing in the acid.

5 . The method of claim 1 , wherein the acid is selected from the group consisting of nitric acid, phosphoric acid, and sulfuric acid.

6 . The method of claim 1 , wherein the acid is nitric acid.

7 . The method of claim 1 , wherein the concentration of the acid is 1N to 10N.

8 . The method of claim 1 , wherein the implant is immersed in the acid for 10 minutes to 60 minutes.

9 . The method of claim 1 , wherein the implant is rinsed with deionized water.

10 . The method of claim 1 , wherein the implant is rinsed for 2 minutes to 10 minutes.

11 . The method of claim 1 , further comprising drying the rinsed implant overnight at room temperature before heating the rinsed implant.

12 . The method of claim 1 , wherein the implant is heated at a temperature of at 400° Celsius to 600° Celsius.

13 . The method of claim 1 , wherein the temperature of the implant during heating is increased from ambient temperature to a predetermined temperature at a rate of 10° Celsius per minute.

14 . The method of claim 1 , wherein the implant is heated to a predetermined temperature between 100° Celsius to 600° Celsius and held at the predetermined temperature for 30 minutes to 60 minutes.

15 . A nano-modified implant prepared by the method of claim 1 .

16 . The nano-modified implant of claim 15 , wherein the surface chemistry of the implant after the heating is the same as the surface chemistry of the implant before immersing the implant with the acid.

17 . The nano-modified implant of claim 15 , wherein bacterial adhesion to the nano-modified implant after heating the implant is less than bacterial adhesion to the implant prior to immersing the implant in the acid.

18 . The nano-modified implant of claim 17 , wherein the bacterial adhesion to the nano-modified implant after heating the implant is 5% to 80% of the bacterial adhesion to the implant prior to immersing the implant in the acid.

19 . The implant of claim 17 , wherein the bacteria is selected from the group consisting of Staphylococcus epidermidis and Staphylococcus aureus.

20 . The nano-modified implant of claim 15 , wherein the average surface roughness is 2 times to 10 times the average surface roughness of the implant prior to immersing the implant in the acid.

21 . The nano-modified implant of claim 15 , wherein the surface of the nano-modified implant contains a plurality of surface features and the average height of the plurality of surface features is 2 times to 10 times the average height of the plurality of surface features of the implant prior to immersing the implant in the acid.

22 . The nano-modified implant of claim 15 , wherein the surface energy of the nano-modified implant after heating the implant is 5% to 80% of the surface energy of the implant prior to immersing the implant in the acid.

23 . A nano-modified implant comprising a surface roughness of 20 nanometers to 80 nanometers.

24 . A nano-modified implant comprising an average surface roughness of 20 nanometers to 80 nanometers; a surface with a plurality of surface features wherein the average width of the plurality of surface features is 1 nanometer to 100 nanometer; and a surface energy as measured in contact angles of 10 degrees to 60 degrees.

25 . A method of decreasing a microbial response of an implant in a mammal, comprising:
a) preparing a site for receipt of an implant prepared by the method of claim 1 in a mammal; and b) inserting the implant into the site.

26 . A method of decreasing a microbial response of an implant in a mammal, comprising:
a) preparing a site for receipt of the implant of claim 24 in a mammal; and b) inserting the implant into the site.

Assignees

• Howmedica Osteonics Corp

Inventors

• Webster Thomas J
• Dimaano Godofredo R
• Tenhuisen Kevor Shane
• Kulesha Gene
• Muth John

Classifications

• A61F2002/3084

  Nanostructures · CPC title

• C23F1/26

  for etching refractory metals · CPC title

• A61L2400/18

  Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment · CPC title

• A61L2400/12

  Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces · CPC title

• A61L31/022 Primary

  Metals or alloys · CPC title