Cell culture kit, screening method, and method of manufacturing cell culture kit

Patent metadata

Field	Value
Publication number	US-2016160175-A1
Application number	US-201615010468-A
Country	US
Kind code	A1
Filing date	Jan 29, 2016
Priority date	Oct 24, 2008
Publication date	Jun 9, 2016
Grant date	—

Abstract

To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers ( 33 ) and living cells derived from various donors are adhered to surfaces of the plurality of microchambers ( 33 ). Specifically, living cells D 1, D 2, and D 3 derived from various donors are adhered to the plurality of microchambers ( 33 ). In each microchamber ( 33 ), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.

First claim

1 . A method of manufacturing a cell culture kit, comprising:
seeding cells in a plurality of micro spaces contained in a plurality of chambers in a cell culture plate, wherein the cells are derived from at least two different human donors; and culturing the seeded living cells in a culture medium,
wherein the cells from the at least two different human donors are adhered to the plurality of micro spaces such that at least one micro space comprises adhered cells from at least two of the different human donors.

2 . The method of claim 1 , wherein at least one micro space adjacent to the microspace that comprises adhered cells from the at least two of the different human donors comprises adhered cells from only one of the different human donors.

3 . The method of claim 1 , wherein at least one micro space adjacent to the micro space that comprises adhered cells from the at least two different human donors further comprises adhered cells from the at least two different human donors.

4 . The method of claim 1 , wherein the cells comprise living cells.

5 . The method of claim 1 , wherein the cells comprise two or more different cell types.

6 . The method of claim 1 , wherein the cells comprise a tissue stem cell.

7 . The kit of claim 1 , wherein the cells comprise at least one selected from the group consisting of a cell differentiated from an ES cell and a cell differentiated from an iPS cell,

8 . The method of claim 1 , wherein the cells are in the form of a three-dimensional structure.

9 . The method of claim 1 , wherein the plurality of micro spaces have a bottom area of 0.01 to 0.1 mm 2 and a depth of 25 to 150 μm.

10 . The method of claim 1 , wherein the cells are present in the plurality of micro spaces at a density of 1×10 2 to 1×10 6 cells/cm 2 .

11 . The method of claim 1 , wherein the cells are in the form of a cell aggregate in each of the plurality of micro spaces.

12 . The method of claim 11 , wherein the cell aggregate has a diameter of 30 to 200 μm.

13 . The method of claim 1 , wherein the cells comprises a tissue precursor cell.

14 . The method of claim 1 , wherein the cells comprises liver cells.

15 . The method of claim 14 , wherein the liver cells comprises a tissue precursor cell.

16 . The method of claim 14 , wherein the liver cells are isolated from hepatic tissues of the at least two different human donors.

17 . The method of claim 1 , wherein the culture medium covers the plurality of micro spaces.

18 . The method of claim 1 , further comprising before seeding the cells, obtaining the cells from at least two of the different human donors.

Assignees

• Kuraray Co
• Public Univ Corp Yokohama City

Inventors

• Itchoda Yoko
• Tazaki Go
• Fukuda Motohiro
• Tsuruta Hitoshi
• Taniguchi Hideki

Classifications

• C12N2503/02

  Drug screening · CPC title

• C12N5/0062 Primary

  General methods for three-dimensional culture · CPC title

• C12N2513/00

  3D culture · CPC title

• C12N2502/00

  Coculture with; Conditioned medium produced by · CPC title

• C12M23/12 Primary

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title