Micro Blood Vessels and Tissue Ducts

Patent metadata

Field	Value
Publication number	US-2015322408-A1
Application number	US-201514789269-A
Country	US
Kind code	A1
Filing date	Jul 1, 2015
Priority date	Jun 9, 2005
Publication date	Nov 12, 2015
Grant date	—

Abstract

A fiber includes one or more layers of polymer surrounding a central lumen, and living animal cells disposed within the lumen and/or within at least one of the one or more layers, wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns. Also disclosed are model tissues including such fibers, and method of making such fibers. The fibers can serve as synthetic blood vessels, ducts, or nerves.

First claim

What is claimed is:

1 . A model tissue comprising:
an inlet port and an outlet port, and a fiber comprising:
one or more layers of polymer surrounding a central lumen, and
living animal cells disposed within the lumen and/or within at least one of the one or more layers,
wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns, and
wherein the fiber is in a condition of having been generated via sheath flow.

2 . The model tissue of claim 1 , further comprising a hydrogel matrix at least partially surrounding said fiber.

3 . The model tissue of claim 1 , wherein said fiber is configured as a blood vessel, tissue duct, or nerve.

4 . The model tissue of claim 1 , comprising a plurality of said fibers.

5 . The model tissue of claim 1 , wherein the fiber is hollow and the lumen is substantially free of polymer.

6 . The model tissue of claim 1 , further comprising cells adhered to an exterior surface of said fiber.

7 . The model tissue of claim 1 , wherein the fiber is free of attachment along a surface.

8 . The model tissue of claim 1 , wherein the polymer in at least one of said one or more layers is biodegradable.

9 . The model tissue of claim 1 , wherein the polymer in at least one of said one or more layers comprises a material is selected from the group consisting of collagen, agarose, polyelectrolytes, chitosan, gelatin, polyethylene glycol, peptides, derivatives thereof, and combinations thereof.

10 . The model tissue of claim 1 , wherein at least one of said one or more layers further comprises a nucleic acid and/or a factor to modify cell growth, adhesion, and/or differentiation.

11 . A model tissue comprising:
an inlet port and an outlet port, and a fiber comprising:
at least two concentric layers of polymer surrounding a central lumen, and
living animal cells disposed within at least one of the at least two concentric layers of polymer and optionally within the lumen, wherein at least two different animal cells are disposed in different layers of polymer and/or lumen,
wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns, and wherein the fiber is in a condition of having been generated via sheath flow.

12 . The model tissue of claim 11 , further comprising a hydrogel matrix at least partially surrounding said fiber.

13 . The model tissue of claim 11 , wherein said fiber is configured as a blood vessel, tissue duct, or nerve.

14 . The model tissue of claim 11 , comprising a plurality of said fibers.

15 . The model tissue of claim 11 , wherein the fiber is hollow and the lumen is substantially free of polymer.

16 . The model tissue of claim 11 , further comprising cells adhered to an exterior surface of said fiber.

17 . The model tissue of claim 11 , wherein the fiber is free of attachment along a surface.

18 . The model tissue of claim 11 , wherein the polymer in at least one of said one or more layers is biodegradable.

19 . The model tissue of claim 11 , wherein the polymer in at least one of said one or more layers comprises a material is selected from the group consisting of collagen, agarose, polyelectrolytes, chitosan, gelatin, polyethylene glycol, peptides, derivatives thereof, and combinations thereof.

20 . The model tissue of claim 11 , wherein at least one of said one or more layers further comprises a nucleic acid and/or a factor to modify cell growth, adhesion, and/or differentiation.

Assignees

• Adams André A
• Daniele Michael
• Ligler Frances S
• Us Government

Inventors

• Adams André A
• Daniele Michael
• Ligler Frances S

Classifications

• B01J2219/00783

  Laminate assemblies, i.e. the reactor comprising a stack of plates · CPC title

• B01L2200/0636

  Focussing flows, e.g. to laminate flows · CPC title

• B01J2219/00889

  Mixing · CPC title

• B01J2219/00891

  Feeding or evacuation · CPC title

• C12M25/12

  the culture medium flowing outside the fiber or tube · CPC title