Method and system for recovering products from an emulsion

Patent metadata

Field	Value
Publication number	US-12576402-B2
Application number	US-202217839589-A
Country	US
Kind code	B2
Filing date	Jun 14, 2022
Priority date	Apr 15, 2016
Publication date	Mar 17, 2026
Grant date	Mar 17, 2026

Abstract

The present invention relates to a method for selecting and recovering products, including providing an emulsion ( 6 ) comprising a plurality of drops ( 4 ) contained in a carrier fluid ( 10 ), each drop comprising an internal fluid ( 8 ), measuring at least one physical parameter for several drops ( 4 ) of the emulsion ( 6 ), classifying at least some of the drops ( 4 ) of the emulsion in a class based on measurements obtained during measuring, tagging at least some of the classified drops ( 4 ) based on the class of the drop ( 4 ), and selectively recovering the drop ( 4 ) or part of the drop ( 4 ) using the tag of the drop or part of the drop ( 4 ).

First claim

What is claimed is:

1 . A method for selecting and recovering products, comprising the following steps:
providing an emulsion comprising a plurality of drops contained in a carrier fluid, each drop comprising an internal fluid, measuring at least one parameter for one or more drops of the emulsion, classifying one or more drops of the emulsion in a class based on one or more measurements obtained during the measuring step, and selectively recovering at least one drop or a part of at least one drop based at least in part on the classified class of the at least one drop.

2 . The method of claim 1 , wherein the measuring step comprises measuring several distinct parameters on one drop.

3 . The method of claim 1 , comprising classifying a plurality of drops of the emulsion simultaneously during the classifying step.

4 . The method of claim 1 , wherein the measuring step comprises measuring at least one parameter reiterated at different successive moments on one drop.

5 . The method of claim 4 , wherein the measuring comprises kinetic reaction monitoring within the one drop.

6 . The method of claim 1 , wherein the internal fluid of one or more drops of the emulsion comprises a signal reagent.

7 . The method of claim 1 , wherein the classifying step comprises classifying the one or more drops into a plurality of classes.

8 . The method of claim 1 , wherein one or more drops of the emulsion comprise at least one cell.

9 . The method of claim 8 , wherein the one or more drops comprise a signal reagent comprising a cell marker.

10 . The method of claim 1 , wherein one or more drops of the emulsion comprise a test reagent able to react with an element within the drop.

11 . The method of claim 10 , wherein the test reagent is fluorescent.

12 . The method of claim 1 , wherein the measuring comprises measuring a result or a product of a test performed in one or more drops.

13 . The method of claim 12 , wherein: (a) the result or product is detected by fluorescence; (b) the test comprises an ELISA test or an immunological test; or both (a) and (b).

14 . The method of claim 1 , wherein the classifying comprises classifying the one or more drops based on one or more criteria selected from the group consisting of secretion rate, antigen affinity, interactions between cells, and cell secretion.

15 . The method of claim 1 , wherein the emulsion is provided as a single layer of drops.

16 . The method of claim 1 , wherein the emulsion is provided as a single layer of drops disposed on a support.

17 . The method of claim 1 , wherein the emulsion is provided as a single layer of drops in a microfluidic chip.

18 . The method of claim 1 , comprising selectively illuminating one or more drops of the emulsion with a light source.

19 . The method of claim 18 , comprising selectively illuminating the one or more drops of the emulsion without illuminating an adjacent drop.

20 . The method of claim 1 , further comprising collecting one or more recovered drops in a tube.

Assignees

• Centre Nat Rech Scient
• Ecole Superieure Physique & Chimie Ind Ville De Paris
• Centre National De La Rech Scientifique—Cnrs

Inventors

• Baudry Jean
• Bibette Jérôme
• Boitard Laurent
• Eyer Klaus
• Langer Krzysztof

Classifications

• G01N33/5094

  for blood cell populations (red blood cells G01N33/80) · CPC title

• C12Q1/6804

  Nucleic acid analysis using immunogens (immunoassay G01N33/53) · CPC title

• B01L2300/168

  Specific optical properties, e.g. reflective coatings · CPC title

• B01L2300/16

  Surface properties and coatings · CPC title

• B01L2300/123

  Flexible; Elastomeric · CPC title