Method and system for recovering products from an emulsion

Patent metadata

Field	Value
Publication number	US-11396017-B2
Application number	US-201716093914-A
Country	US
Kind code	B2
Filing date	Apr 14, 2017
Priority date	Apr 15, 2016
Publication date	Jul 26, 2022
Grant date	Jul 26, 2022

Abstract

The present invention relates to a method for selecting and recovering products, comprising the following steps:providing an emulsion (6) comprising a plurality of drops (4) contained in a carrier fluid (10), each drop comprising an internal fluid (8),measuring at least one physical parameter for several drops (4) of the emulsion (6),classifying at least some of the drops (4) of the emulsion in a class based on measurements obtained during the measuring step,tagging at least some of the classified drops (4) based on the class of the drop (4),selectively recovering the drop (4) or part of the drop (4) using the tag of the drop or part of the drop (4).

First claim

The invention claimed is:

1. A method for selecting and recovering products comprising:
providing an emulsion comprising a plurality of drops contained in a carrier fluid, each drop comprising an internal fluid,
measuring at least one physical parameter for several drops of the emulsion,
classifying at least some of the drops of the emulsion in a class based on measurements obtained during the measuring,
tagging at least some of the classified drops based on the class of the drop, and
selectively recovering the drop or part of the drop using the tag of the drop or part of the drop;
wherein the internal fluid of each drop comprises a signal reagent, and the tagging comprises optical activation of the signal reagent depending on the classification of the drop.

2. The method according to claim 1 , wherein a plurality of drops are classified simultaneously during the classification, and the selective recovery includes sorting the drops after tagging each classified drop.

3. The method according to claim 1 , wherein the measuring comprises measuring at least one parameter reiterated at different successive moments on the same drops.

4. The method according to claim 1 , wherein for each drop of a plurality of drops, the measuring comprises measuring several distinct parameters on the same drop.

5. The method according to claim 1 , wherein a plurality of drops are classified during the classification in a plurality of classes, the tagging comprising illuminating each drop from a class with preset illumination conditions, the signal reagent being able to emit a light signal, advantageously fluorescence, after its activation, the drops being sorted based on their light signal level emitted during the selective recovery, the level of the light signal emitted by the signal reagent depending on illumination conditions during the activation.

6. The method according to claim 1 , wherein the internal fluid of each drop comprises a signal reagent, and the tagging comprises thermal activation of the signal reagent depending on the classification of the drop.

7. The method according to claim 1 , wherein the emulsion is provided in a two-dimensional chamber, the emulsion being kept in the form of a single layer of drops in the two-dimensional chamber during the measuring and the tagging.

8. The method according to claim 1 , wherein a classified drop includes a cell, the tagging comprises marking the cell, and the selective recovery comprises an emulsion break followed by sorting by cytometry.

9. A system for selecting and recovering products comprising:
a support able to receive an emulsion comprising a plurality of drops contained in a carrier fluid, each drop comprising an internal fluid,
a device for measuring at least one physical parameter for several drops of the emulsion received in the support,
a classifying unit able to decide the classification of at least one drop of the emulsion based on measurements obtained during measuring,
a tagging device able to tag a drop or part of the drop based on the classification decision of the drop, and
a recovery device of the drop or part of the drop based on the tagging,
wherein the internal fluid of each drop comprises a signal reagent, and the tagging device is capable of performing an optical activation of the signal reagent depending on the classification of the drop.

10. The method according to claim 1 , wherein a plurality of drops in the carrier fluid is stored in a two-dimensional chamber during measuring and the tagging, and the drops are arranged in the chamber so that no drop is superimposed on another drop.

11. A method for selecting and recovering products comprising:
providing an emulsion comprising a plurality of drops contained in a carrier fluid, each drop comprising an internal fluid,
measuring at least one physical parameter for several drops of the emulsion,
classifying at least some of the drops of the emulsion in a class based on measurements obtained during the measuring,
tagging at least some of the classified drops based on the class of the drop, and
selectively recovering the drop or part of the drop using the tag of the drop or part of the drop,
wherein the emulsion is provided in a two-dimensional chamber, the emulsion being kept in the form of a single layer of drops in the two-dimensional chamber during the measuring and the tagging.

12. A system for selecting and recovering products comprising:
a support able to receive an emulsion comprising a plurality of drops contained in a carrier fluid, each drop comprising an internal fluid,
a device for measuring at least one physical parameter for several drops of the emulsion received in the support,
a classifying unit able to decide the classification of at least one drop of the emulsion based on measurements obtained during measuring,
a tagging device able to tag a drop or part of the drop based on the classification decision of the drop, and
a recovery device of the drop or part of the drop based on the tagging,
wherein the emulsion is provided in a two-dimensional chamber, the emulsion being kept in the form of a single layer of drops in the two-dimensional chamber during the measuring and the tagging.

13. The method according to claim 3 , further comprising kinetic reaction monitoring within each drop.

Assignees

• Ecole Superieure Physique & Chimie Ind Ville De Paris

Inventors

• Baudry Jean
• Bibette Jérôme
• Boitard Laurent
• Eyer Klaus
• Langer Krzysztof

Classifications

• G01N2015/003

  in liquids, e.g. emulsion · CPC title

• G01N15/1484 Primary

  microstructural devices · CPC title

• G01N15/1459

  the analysis being performed on a sample stream · CPC title

• G01N15/1456

  without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals · CPC title

• B01L2300/168

  Specific optical properties, e.g. reflective coatings · CPC title