Host cells and methods of use
US-9926570-B2 · Mar 27, 2018 · US
Fusion polypeptides
US12559759B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12559759-B2 |
| Application number | US-202017437189-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 9, 2020 |
| Priority date | Mar 8, 2019 |
| Publication date | Feb 24, 2026 |
| Grant date | Feb 24, 2026 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
Provided herein, inter alia, are fusion DNA constructs comprising improved protease recognition sequences for expressing and purifying one or more polypeptides of interest as well as methods for producing one or more polypeptides of interest in a recombinant host cell.
First claim
Opening claim text (preview).
We claim: 1 . A fusion polypeptide comprising a KEX2 region, wherein the amino acid sequence of the KEX region comprises TKVAVEKRQV (SEQ ID NO:10), wherein the amino acid sequence is completely cleaved by one or more protease(s) and wherein the protease is a Kex2 serine peptidase (EC 3.4.21.61). 2 . The fusion polypeptide of claim 1 , further comprising a polypeptide encoding a signal sequence. 3 . The fusion polypeptide of claim 2 , wherein the polypeptide encoding a signal sequence is located N-terminal or C-terminal from the amino acid sequence of the KEX region. 4 . The fusion polypeptide of claim 1 , further comprising a polypeptide encoding a carrier protein. 5 . The fusion polypeptide of claim 4 , wherein the polypeptide encoding a carrier protein is located N-terminal or C-terminal from the amino acid sequence of the KEX region. 6 . The fusion polypeptide of claim 5 , wherein the polypeptide encoding a carrier protein is adjacent to the polypeptide encoding a signal sequence. 7 . The fusion polypeptide of claim 5 , wherein the carrier protein comprises CBH1 or a fragment thereof.
Assignees
Inventors
Classifications
- C12N9/60Primary
from yeast · CPC title
containing protease site · CPC title
- C07K16/11Primary
Paramyxoviridae (F); Pneumoviridae (F), e.g. respiratory syncytial virus [RSV] · CPC title
Specific host cells or culture conditions, e.g. components, pH or temperature · CPC title
containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US12559759B2 cover?
- Provided herein, inter alia, are fusion DNA constructs comprising improved protease recognition sequences for expressing and purifying one or more polypeptides of interest as well as methods for producing one or more polypeptides of interest in a recombinant host cell.
- Who is the assignee on this patent?
- Danisco Us Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/60. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 24 2026 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).