Processes of producing fermentation products

Patent metadata

Field	Value
Publication number	US-12540340-B2
Application number	US-202117177773-A
Country	US
Kind code	B2
Filing date	Feb 17, 2021
Priority date	Mar 30, 2012
Publication date	Feb 3, 2026
Grant date	Feb 3, 2026

Abstract

The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.

First claim

The invention claimed is:

1 . A process for producing ethanol from a starch-containing material comprising corn, comprising the steps of:
i) liquefying the starch-containing material at a temperature between 70-95 degrees centigrade using an alpha-amylase; ii) saccharifying using a glucoamylase; iii) fermenting using a yeast fermenting microorganism to produce the ethanol; and
wherein a cellulolytic composition comprising an endoglucanase, a beta-glucosidase, and a cellobiohydrolase is added during saccharification or fermentation, wherein ii) saccharifying and iii) fermenting are done simultaneously, and the beta-glucosidase has at least 90% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6, comprises one or more substitutions selected from the group consisting of L89M, G91L, F100D, I140V, I186V, S283G, and N456E, and has a Relative ED50 loading value of less than 1, and wherein the ethanol yield is increased compared to the ethanol yield obtained without the cellulolytic composition and wherein sequence identity is determined using the Needleman-Wunsch algorithm as implemented in the Needle program of the EMBOSS package, version 5.0.0, using a gap open penalty of 10, a gap extension penalty of 0.5, and EBLOSUM62 substitution matrix.

2 . The process of claim 1 , wherein the pH during liquefaction is from 4-7.

3 . The process of claim 1 , wherein the cellulolytic composition is derived from a strain of Trichoderma , a strain of Humicola , or a strain of Chrysosporium.

4 . The process of claim 1 , wherein the yeast is a Saccharomyces sp. yeast.

5 . The process of claim 1 , wherein the yeast is a Saccharomyces cerevisiae yeast.

6 . The process of claim 1 , wherein the cellobiohydrolase is cellobiohydrolase I (CBHI).

7 . The process of claim 1 , wherein the cellobiohydrolase is a cellobiohydrolase II (CBHII).

8 . The process of claim 1 , further wherein a trehalase is added during simultaneous saccharification and fermentation.

9 . The process of claim 1 , wherein the beta-glucosidase has at least 90% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6.

10 . The process of claim 1 , wherein the beta-glucosidase has at least 92% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6.

11 . The process of claim 1 , wherein the beta-glucosidase has at least 93% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6.

12 . The process of claim 1 , wherein the beta-glucosidase has at least 94% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6.

13 . The process of claim 1 , wherein the beta-glucosidase has at least 95% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6.

14 . The process of claim 1 , wherein the beta-glucosidase has at least 96% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6.

15 . The process of claim 1 , wherein the beta-glucosidase has at least 97% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6.

16 . The process of claim 1 , wherein the beta-glucosidase has at least 98% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6.

17 . The process of claim 1 , wherein the beta-glucosidase has at least 99% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6.

18 . The process of claim 1 , wherein the beta-glucosidase comprises 1, 2, 3, or 4 substitutions selected from the group consisting of L89M, G91L, F100D, I140V, I186V, S283G, and N456E.

19 . The process of claim 18 , wherein the beta-glucosidase further comprises a F512Y substitution.

20 . The process of claim 1 , wherein the beta-glucosidase further comprises a F512Y substitution.

Assignees

• Novozymes As

Inventors

• Rasmussen Thomas
• Saunders Jeremy
• Croonenberghs James
• Kang Zhengfang
• Craig Joyce
• Akerman Michael John

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title

• C12N9/2445

  Beta-glucosidase (3.2.1.21) · CPC title

• C12P7/14 Primary

  Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms · CPC title