Omentum based scaffold and delivery system

Patent metadata

Field	Value
Publication number	US-12514957-B2
Application number	US-201916686158-A
Country	US
Kind code	B2
Filing date	Nov 17, 2019
Priority date	Jun 24, 2013
Publication date	Jan 6, 2026
Grant date	Jan 6, 2026

Abstract

Compositions of matter comprising decellularized omentum are disclosed. The compositions may be scaffolds, hydrogels or hydrogel precursor compositions. Methods of generating the compositions are disclosed as well as uses thereof.

First claim

What is claimed is:

1 . A method of 3D printing a 3D structure of cardiac tissue, the method comprising:
(a) digesting decellularized omentum with a proteolytic enzyme to generate a liquid composition which comprises solubilized, decellularized omentum, said liquid composition being capable of forming a gel at 37° C.;
wherein the liquid composition is more than 99% devoid of nucleic acid or residual nucleic acid components and wherein the liquid composition is more than 99% devoid of lipid components;
(b) combining said liquid composition with viable cardiac cells to form a mixture comprising solubilized, decellularized omentum and the viable cardiac cells; and (c) extruding said mixture through an aperture; and (d) generating 3D structure of cardiac tissue comprising viable cardiac cells having an elongated morphology and decellularized omentum, thereby 3D printing the 3D structure of cardiac tissue;
further comprising decellularizing said omentum prior to said digesting wherein said decellularizing comprises:
(i) exposing the omentum to a hypotonic solution to produce hypotonic shock; (ii) exposing the omentum to freeze-thaw conditions following step (i); (iii) dehydrating said omentum following step (ii); (iv) extracting fat from the dehydrated omentum using polar and non-polar extraction agents following step (iii); (v) rehydrating the dehydrated omentum following step (iv); and (vi) extracting cells from said rehydrated omentum following step (v); wherein step (iv) also comprises washing the dehydrated omentum multiple times with acetone, followed by three incubations in a 60:40 hexane:acetone solution.

2 . The method of claim 1 , wherein said omentum is human omentum.

3 . The method of claim 1 , wherein a diameter of said aperture is between 0.1-0.7 mm.

4 . The method of claim 1 , wherein the mixture consists of viable cells and decellularized omentum.

5 . The method of claim 1 , further comprising combining said liquid composition with endothelial cells following step (a) and prior to step (c).

6 . The method of claim 5 , wherein said 3D structure of cardiac tissue comprises blood vessels.

Assignees

• Univ Ramot

Inventors

• Dvir Tal
• Peer Dan
• Shevach Michal
• Soffer Tsur Neta

Classifications

• C12N5/0696

  Artificially induced pluripotent stem cells, e.g. iPS · CPC title

• C12N5/0691

  Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels · CPC title

• C12N5/0657

  Cardiomyocytes; Heart cells · CPC title

• A61L2430/40

  Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking · CPC title

• A61L27/3826

  Muscle cells, e.g. smooth muscle cells · CPC title