Particles comprising decellularized omentum

Patent metadata

Field	Value
Publication number	US-11213609-B2
Application number	US-201616061675-A
Country	US
Kind code	B2
Filing date	Dec 15, 2016
Priority date	Dec 16, 2015
Publication date	Jan 4, 2022
Grant date	Jan 4, 2022

Abstract

A spherical particle comprising decellularized omentum being between 1 nM-300 μM in diameter is disclosed. In some embodiments, the particle comprises biological cells. In other embodiments, the particle comprises a biomolecule. Uses of the particles are also disclosed.

First claim

What is claimed is:

1. A composition comprising a plurality of spherical particles being between 50-300 μm in diameter, wherein said spherical particles encapsulate biological cells, wherein said spherical particles consist of decellularized omentum and biological cells.

2. The composition of claim 1 , wherein said omentum comprises human omentum.

3. The composition of claim 1 , wherein said biological cells are selected from the group consisting of cardiac cells, neuronal cells, pancreatic cells, stem cells, liver cells, muscle cells, blood cells and immune cells.

4. The composition of claim 1 , wherein each of the particles of said plurality of particles are of substantially the same size.

5. The composition of claim 1 , which does not induce an immunological response when implanted in a subject, when said omentum is autologous to said subject.

6. The composition of claim 1 , wherein said biological cells comprise cardiomyocytes.

7. The composition of claim 1 , wherein said spherical particles are between 100-300 μm in diameter.

8. The composition of claim 1 , being generated by a method comprising:
(a) dispersing a composition comprising solubilized decellularized omentum and biological cells in a mixture comprising a fluorinated oil and surfactant, under conditions that allow generation of droplets of emulsified, decellularized omentum;
(b) heating said droplets of emulsified, decellularized omentum to generate solid particles which consist of decellularized omentum and biological cells; and
(c) separating said solid particles consisting of decellularized omentum from said mixture following said heating, wherein said method is effected using a microfluidics device.

9. A method of treating a disease or medical condition which would benefit from cell transplantation in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a plurality of the particles of claim 1 , thereby treating the medical condition.

Assignees

• Univ Ramot

Inventors

• Dvir Tal
• Shapira Assaf
• Shevach Michal
• Gal Idan

Classifications

• A61L27/3604 Primary

  characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel · CPC title

• A61L27/3826

  Muscle cells, e.g. smooth muscle cells · CPC title

• A61L2430/40

  Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• A61L27/3804

  characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells · CPC title