Oilseed plants having reduced pod shatter

Patent metadata

Field	Value
Publication number	US-12507650-B2
Application number	US-202318137263-A
Country	US
Kind code	B2
Filing date	Apr 20, 2023
Priority date	Aug 18, 2017
Publication date	Dec 30, 2025
Grant date	Dec 30, 2025

Abstract

This document relates to materials and methods for domesticating oilseed (e.g., pennycress) plants. For example, oilseed plants having reduced seedpod shatter, as well as materials and methods for making and using oilseed plants having reduced seedpod shatter are provided.

First claim

What is claimed is:

1 . A pennycress plant comprising a modification in an indehiscent (IND) gene, wherein an unmodified IND gene encodes a polypeptide having at least 95% sequence identity to SEQ ID NO:9, wherein the plant comprising the modified IND gene exhibits reduced seedpod shatter compared to a corresponding plant lacking the modification.

2 . The plant of claim 1 , wherein the modification comprises a substitution.

3 . The plant of claim 1 , wherein
the modified IND gene comprises a C to T substitution at position 247 relative to SEQ ID NO:11, and the modified IND polypeptide comprises SEQ ID NO:12; the modified IND gene comprises a G to A substitution at position 260 relative to SEQ ID NO:13, and the modified IND polypeptide comprises SEQ ID NO:14; or the modified IND gene comprises a G to A substitution at position 301 relative to SEQ ID NO:15, and the modified IND polypeptide comprises SEQ ID NO: 16.

4 . The plant of claim 1 , wherein the plant comprises seedpods that require about 20 grams to about 120 grams of pulling force to shatter.

5 . The plant of claim 1 , wherein the plant comprises seedpods that are resistant to shatter under less than about 30 grams force.

6 . Seed produced by the plant of claim 1 .

7 . A method for generating a modified pennycress plant having reduced seedpod shatter compared to a corresponding pennycress plant lacking the modification, the method comprising:
modifying an indehiscent (IND) gene in the pennycress genome, wherein an unmodified IND gene encodes a polypeptide having at least 95% sequence identity to SEQ ID NO: 9, generating a modified pennycress plant, wherein the modified plant exhibits reduced seedpod shatter compared to a corresponding pennycress plant lacking the modification.

8 . The method of claim 7 , wherein the modifying comprises site-specific gene editing, wherein the site-specific gene editing comprising introducing a guide sequence and a nuclease into a plant cell, wherein the guide sequence comprises a nucleic acid sequence specific to the IND gene, and wherein the nuclease is a CRISPR associated system (Cas) nuclease.

9 . The method of claim 7 , wherein the modifying comprises mutagenesis, wherein the mutagenesis comprises introducing a mutagen into a plant cell, wherein the mutagen comprises ethyl methane sulphonate (EMS).

10 . The method of claim 7 , wherein the modifying comprises a substitution.

11 . The method of claim 7 , wherein
the modified IND gene comprises a C to T substitution at position 247 relative to SEQ ID NO:11, and the modified IND polypeptide comprises SEQ ID NO:12; the modified IND gene comprises a G to A substitution at position 260 relative to SEQ ID NO:13, and the modified IND polypeptide comprises SEQ ID NO:14; or the modified IND gene comprises a G to A substitution at position 301 relative to SEQ ID NO:15, and the modified IND polypeptide comprises SEQ ID NO:16.

12 . The method of claim 7 , further comprising:
selecting a plant cell having a modification in the IND gene; and regenerating a modified plant from the selected plant cell, wherein the plant exhibits reduced seedpod shatter.

13 . The method of claim 7 , wherein the modified plant comprises seedpods that require about 20 grams to about 120 grams of pulling force to shatter.

14 . The method of claim 7 , wherein the modified plant comprises seedpods that are resistant to shatter under less than about 30 grams force.

Assignees

• Univ Minnesota
• The Board Of Trustees Of Illinois State Univ

Inventors

• Marks Michael David
• Sedbrook John C
• Chopra Ratan
• Esfahanian Maliheh

Classifications

• C12Y302/01015

  Polygalacturonase (3.2.1.15) · CPC title

• C12N9/2402

  hydrolysing O- and S- glycosyl compounds (3.2.1) · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8266

  Abscission; Dehiscence; Senescence · CPC title

• C12N15/8213

  Targeted insertion of genes into the plant genome by homologous recombination · CPC title