Methods and compositions for the introduction and regulated expression of genes in plants
US-11512321-B2 · Nov 29, 2022 · US
Methods and compositions for the introduction and regulated expression of genes in plants
US12497628B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12497628-B2 |
| Application number | US-202218047714-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 19, 2022 |
| Priority date | Dec 30, 2009 |
| Publication date | Dec 16, 2025 |
| Grant date | Dec 16, 2025 |
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Abstract
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Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
First claim
Opening claim text (preview).
That which is claimed: 1 . A method for introducing by Agrobacterium -mediated transformation of a polynucleotide of interest into a monocot leaf tissue explant, said method comprising: a) providing the monocot leaf tissue explant, wherein the leaf tissue explant is obtained from a leaf segment excised from a leaf above the leaf base node; b) incubating the leaf segments of the leaf tissue explant with Agrobacterium comprising the polynucleotide of interest; c) introducing into said monocot leaf segments of the leaf tissue explant: i) a heterologous polynucleotide encoding a Babyboom cell proliferation factor and a heterologous polynucleotide encoding a Wuschel cell proliferation factor; and expressing said heterologous polynucleotide encoding said Babyboom cell proliferation factor and said heterologous polynucleotide encoding said Wuschel cell proliferation factor; and ii) a polynucleotide of interest; and d) regenerating a monocot plant comprising the polynucleotide of interest. 2 . The method of claim 1 , further comprising excising said heterologous polynucleotide encoding said Babyboom cell proliferation factor and said heterologous polynucleotide encoding said Wuschel cell proliferation factor. 3 . The method of claim 1 , wherein the monocot is selected from the group consisting of maize, sorghum, sugarcane, rice, and wheat.
Assignees
Inventors
Classifications
involving plant development · CPC title
Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title
- C12N15/8213Primary
Targeted insertion of genes into the plant genome by homologous recombination · CPC title
- C12N15/8241Primary
Phenotypically and genetically modified plants via recombinant DNA technology · CPC title
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- What does patent US12497628B2 cover?
- Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and pla…
- Who is the assignee on this patent?
- Corteva Agriscience Llc, Pioneer Hi Bred Int
- What technology area does this patent fall under?
- Primary CPC classification C12N15/8213. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 16 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).