Methods and compositions for the introduction and regulated expression of genes in plants

Patent metadata

Field	Value
Publication number	US-11512321-B2
Application number	US-202117181136-A
Country	US
Kind code	B2
Filing date	Feb 22, 2021
Priority date	Dec 30, 2009
Publication date	Nov 29, 2022
Grant date	Nov 29, 2022

Abstract

Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.

First claim

That which is claimed:

1. A method for introducing a polynucleotide of interest into a maize leaf tissue explant, said method comprising:
a) excising a leaf segment from a leaf above the first leaf base node;
b) dissecting said leaf segment into a leaf tissue explant;
c) introducing into said leaf tissue explant: i) a heterologous polynucleotide encoding a babyboom cell proliferation factor and a heterologous polynucleotide encoding a Wuschel cell proliferation factor; and expressing said heterologous polynucleotide encoding said babyboom cell proliferation factor and said heterologous polynucleotide encoding said Wuschel cell proliferation factor; and ii) a polynucleotide of interest; and
d) regenerating a monocot plant comprising the polynucleotide of interest.

2. The method of claim 1 , further comprising excising said heterologous polynucleotide encoding said babyboom cell proliferation factor and said heterologous polynucleotide encoding said Wuschel cell proliferation factor.

Assignees

• Pioneer Hi Bred Int
• Du Pont

Inventors

• Gordon-Kamm William James
• Klein Theodore M
• Lowe Keith S
• Mcbride Kevin E
• Scelonge Christopher J
• Wang Ning

Classifications

• C12N15/8262

  involving plant development · CPC title

• C12N15/8216

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8213 Primary

  Targeted insertion of genes into the plant genome by homologous recombination · CPC title

• C12N15/8241 Primary

  Phenotypically and genetically modified plants via recombinant DNA technology · CPC title