Cell treatment device, suspension culture vessel, and stem cell induction method

Patent metadata

Field	Value
Publication number	US-12486485-B2
Application number	US-202217934953-A
Country	US
Kind code	B2
Filing date	Sep 23, 2022
Priority date	Feb 24, 2017
Publication date	Dec 2, 2025
Grant date	Dec 2, 2025

Abstract

This cell treatment device is provided with: a factor introduction device 30 for introducing a pluripotent induction factor into cells so as to prepare induction factor-introduced cells; and a reprogramming suspension culture vessel for culturing the induction factor-introduced cells that have been prepared by the factor introduction device 30.

First claim

The invention claimed is:

1 . A method for manufacturing pluripotent stem cells comprising:
introducing a pluripotency inducing factor into cells to create inducing factor-introduced cells by a factor introducer; transferring the inducing factor-introduced cells from the factor introducer to a suspension culture vessel through an introduced cell solution-feeding channel, wherein the introduced cell solution-feeding channel is configured to send a solution that contains the inducing factor-introduced cells from the factor introducer to the suspension culture vessel, the suspension culture vessel comprises a container and a semipermeable membrane in the form of a tube inside the container, and the inducing factor-introduced cells are transferred from the factor introducer to inside of the tube of the semipermeable membrane; suspension culturing the inducing factor-introduced cells in the tube of the semipermeable membrane in the suspension culture vessel, until pluripotent stem cells are induced from the inducing factor-introduced cells and a colony of the induced pluripotent stem cells is formed; and agitating a culture medium in the container of the suspension culture vessel by an agitator only from outside of the tube of the semipermeable membrane.

2 . The method according to claim 1 , wherein the inducing factor-introduced cells are reprogrammed inside the suspension culture vessel.

3 . The method according to claim 1 , wherein the induced cells are amplifying cultured inside the suspension culture vessel.

4 . The method according to claim 1 , further comprising: introducing culture medium outside the tube of the semipermeable membrane and inside the container.

5 . The method according to claim 1 ,
wherein the suspension culture vessel further comprises a drive unit, and in the agitating, the agitator is rotated by the drive unit.

6 . The method according to claim 1 , wherein in the agitating, the culture medium in the container is agitated such that a flow rate of the culture medium inside the tube of the semipermeable membrane is slower than a flow rate of the culture medium outside the tube of the semipermeable membrane.

7 . A method for manufacturing pluripotent stem cells comprising:
preparing a suspension culture vessel comprising
a container,
a semipermeable membrane in the form of a tube placed in the container,
a first solution-feeding channel connected to the container,
an agitator provided outside the tube of the semipermeable membrane and inside the container, and
a second solution-feeding channel connected to the container;
introducing a culture medium containing an inducing factor-introduced cells into the tube of the semipermeable membrane through the first solution-feeding channel; introducing a culture medium in the container around a periphery of the semipermeable membrane; suspension-culturing the inducing factor-introduced cells in the tube of the semipermeable membrane until pluripotent stem cells are induced from the inducing factor-introduced cells and a colony of the pluripotent stem cells is formed; agitating the culture medium only from outside of the tube of the semipermeable membrane and inside the container by the agitator; and transferring the induced pluripotent stem cells from the tube of the semipermeable membrane to an outside of the container through the second solution-feeding channel.

8 . The method according to claim 7 , further comprising:
driving the agitator by a drive unit provided outside the container by magnetic force.

9 . The method according to claim 7 , wherein in the agitating, the culture medium is agitated such that a flow rate of the culture medium inside the tube of the semipermeable membrane is slower than a flow rate of the culture medium outside the tube of the semipermeable membrane.

Assignees

• I Peace Inc
• Tanabe Koji

Inventors

• Tanabe Koji
• Suto Kenta
• Hiraide Ryoji

Classifications

• C12N5/0606

  Pluripotent embryonic cells, e.g. embryonic stem cells [ES] (embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696) · CPC title

• C12M29/00

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title

• C12M23/02

  Form or structure of the vessel (large containers B65D88/00) · CPC title

• C12N5/10

  Cells modified by introduction of foreign genetic material · CPC title

• C12M41/36 Primary

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title