Cell culturing device

Patent metadata

Field	Value
Publication number	US-2016355774-A1
Application number	US-201515118942-A
Country	US
Kind code	A1
Filing date	Feb 17, 2015
Priority date	Feb 17, 2014
Publication date	Dec 8, 2016
Grant date	—

Abstract

A cell culturing device includes a culture tank, a shaft member at least partially disposed in the culture tank, a stirring mechanism supported by the shaft member, disposed in the culture tank, and a filter disposed in contact with the shaft member so as to suck the culture solution from the culture tank and/or to supply the culture solution to the culture tank. The shaft member is at least partially hollow, has openings to introduce the culture solution to the interior or to discharge the culture solution from the interior, and is unrotatable. Suction of the culture solution from the culture tank and/or supply of the culture solution to the tank is performed through the filter and the interior of the shaft member.

First claim

1 . A cell culturing device comprising:
a culture tank configured to accommodate culture solution containing a cell; a shaft member at least partially disposed in the culture tank; stirring means supported by the shaft member and disposed in the culture tank, the stirring means having a stirring blade configured to be rotatable about the shaft member; and a filter disposed in contact with the shaft member, the filter allowing the culture solution to pass through and not allowing the cell to pass through, wherein the shaft member is at least partially hollow, has an opening to introduce the culture solution to interior of the shaft member or to discharge the culture solution from the interior, and is configured to be unrotatable, the filter is located inner than a movable region of the stirring blade rotating about the shaft member and is disposed on the shaft member independently of the stirring blade, and suction of the culture solution from the culture tank and/or supply of the culture solution to the culture tank is performed through the filter and the interior of the shaft member.

2 . The cell culturing device according to claim 1 , wherein the shaft member is supported by a bottom of the culture tank.

3 . The cell culturing device according to claim 2 , wherein
the shaft member has a lower end having the opening and has an upper end closed, the shaft member has a side surface having an opening in communication with the interior, and the filter is disposed on an outer surface of the shaft member so as to cover the opening at the side surface.

4 . The cell culturing device according to claim 3 , wherein the filter has a tubular shape and extends on outside of the shaft member so as to cover the opening at the side surface.

5 . The cell culturing device according to claim 2 , wherein
the shaft member has a tubular shape that has a lower end having the opening and that has an upper end having an opening in communication with the interior, and the filter is disposed on the shaft member so as to cover the opening at the upper end.

6 . The cell culturing device according to claim 5 , wherein the filter has a tubular shape with a base and extends on outside of the shaft member so as to cover the opening at the upper end.

7 . The cell culturing device according to claim 1 , wherein the shaft member is supported by a lid of the culture tank.

8 . The cell culturing device according to claim 7 , wherein
the shaft member has a tubular shape that has an upper end having the opening and that has a lower end having an opening in communication with the interior, and the filter is disposed on the shaft member so as to cover the opening at the lower end.

9 . The cell culturing device according to claim 8 , wherein the filter has a tubular shape with a base and extends on outside of the shaft member so as to cover the opening at the lower end.

10 . The cell culturing device according to claim 7 , wherein
the shaft member has an upper end having the opening and has a lower end closed, the shaft member has a side surface having an opening in communication with the interior, and the filter is disposed on an outer surface of the shaft member so as to cover the opening at the side surface.

11 . The cell culturing device according to claim 10 , wherein the filter has a tubular shape and extends on outside of the shaft member so as to cover the opening at the side surface.

12 . The cell culturing device according to any one of claims claim 1 , wherein the filter is porous.

Assignees

• Asahi Chemical Ind
• Tokyo Women'S Medical Univ

Inventors

• Konishi Kanako
• Iwamoto Ushio
• Wada Masanori
• Matsuura Katsuhisa
• Shimizu Tatsuya
• Okano Teruo

Classifications

• C12M23/08 Primary

  Flask, bottle or test tube · CPC title

• C12M23/38

  Caps; Covers; Plugs; Pouring means · CPC title

• C12M27/02 Primary

  Stirrer or mobile mixing elements · CPC title

• C12M29/04

  Filters; Permeable or porous membranes or plates, e.g. dialysis · CPC title

• C12M33/04

  by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02) · CPC title