Method of improving biomass high-solid enzymatic hydrolysis rate

Patent metadata

Field	Value
Publication number	US-12467072-B2
Application number	US-202118023067-A
Country	US
Kind code	B2
Filing date	Nov 30, 2021
Priority date	Dec 4, 2020
Publication date	Nov 11, 2025
Grant date	Nov 11, 2025

Abstract

The present invention provides a method of improving a biomass high-solid enzymatic hydrolysis rate. By screening particle sizes of the biomass substrate, different water content controls are performed on coarse and fine particle biomass substrates and then an entire water content of the mixture of the coarse and fine particle biomass substrates is controlled to achieve a reduced initial viscosity of the enzymatic hydrolysis and improve liquefaction speed, and finally improve the biomass high-solid enzymatic hydrolysis rate. The enzymatic hydrolysis system of the present invention can be easily constructed with lower equipment requirements, lower energy consumption, higher enzymatic hydrolysis efficiency and simpler operation process, helping the industrial production and applications.

First claim

The invention claimed is:

1 . A method of improving a biomass high-solid enzymatic hydrolysis rate, comprising the following steps:
at step 1, obtaining a biomass substrate with a water content of 50% to 70% by weight by performing pretreatment on a biomass raw material, and screening the biomass substrate through a mesh screen to obtain a coarse-particle biomass substrate above 10 meshes and a fine-particle biomass substrate from 10 to 50 meshes; at step 2, adjusting a water content of the fine-particle biomass substrate to 60% to 65% by weight and a water content of the coarse-particle biomass substrate to 50% to 70% by weight, and mixing the fine-particle biomass substrate and coarse-particle biomass substrate having different water contents to obtain a mixed substrate having a water content of 50% to 65% by weight; at step 3, placing the mixed substrate into an enzymatic hydrolysis tank, and then adding a citrate buffer solution with pH 4.6 to 5.5 and a cellulase for enzymatic hydrolysis.

2 . The method of claim 1 , wherein in step 3, a dry matter mass of the added mixed substrate is 15% to 25%.

3 . The method of claim 1 , wherein, in step 3, an amount of the added cellulase is 4%-6% of a dry matter mass in an enzymatic hydrolysis system.

4 . The method of claim 1 , wherein, in step 3, the added cellulase is Cellic CTec2.

5 . The method of claim 1 , wherein, in step 3, a control temperature of the enzymatic hydrolysis tank is 45° C. to 50° C., a stirring speed of an stirring apparatus in the enzymatic hydrolysis tank is 50 rpm to 300 rpm, and an enzymatic hydrolysis time is 48 h to 96 h.

Assignees

• Zhejiang Huakang Pharmaceutical Co Ltd
• Univ Zhejiang Technology

Inventors

• Li Mian
• Liu Zhiqian
• Zeng Xuhao
• Hu Changhui
• Wang Jing
• Cai Xue
• Zhang Xiaojian
• Zheng Yuguo

Classifications

• C12Y302/01004

  Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title

• C12P2201/00

  Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis · CPC title

• C12N9/2437

  Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150) · CPC title

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title