Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars

Patent metadata

Field	Value
Publication number	US-11421256-B2
Application number	US-201816163196-A
Country	US
Kind code	B2
Filing date	Oct 17, 2018
Priority date	Oct 8, 2009
Publication date	Aug 23, 2022
Grant date	Aug 23, 2022

Abstract

The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps:a) optionally pre-treatmentb) optionally washing;c) enzymatic hydrolysis;d) fermentation; ande) optionally recovery of a fermentation product;wherein in step c) an enzyme composition is used that has a temperature optimum of 55 degrees C. or more, the hydrolysis time is 40 hours or more and the temperature is 50 degrees C. or more.

First claim

The invention claimed is:

1. A process for preparing a biofuel from corn fiber, corn stover or wheat straw comprising:
a) pre-treating the corn fiber, corn stover or wheat straw;
b) enzymatically hydrolyzing the corn fiber, corn stover or wheat straw to produce a hydrolysate;
c) fermenting the hydrolysate into a biofuel; and
d) recovering the biofuel;
wherein enzymes used in step b) comprise of a broth resulting from growth of a filamentous fungal strain in a medium, which have a temperature optimum of 50-70° C.;
wherein a dry matter content of said corn fiber, corn stover or wheat straw in step b) is about 10-33% by weight;
wherein step b) is performed at a pH from about 4-6;
wherein step b) results in a release of at least 70% of available sugar in the corn fiber, corn stover or wheat straw;
wherein step b) occurs from 40-130 hours at a temperature of 50-70° C.; and
wherein in step c), the fermentation is conducted with a yeast strain that is able to ferment at least one C5 sugar selected from the group consisting of arabinose and xylose.

2. The process of claim 1 which uses said corn stover.

3. The process of claim 1 which uses said wheat straw.

4. The process of claim 1 which uses said corn fiber.

5. The process of claim 1 , wherein step b) is performed for about 72 to 120 hours.

6. The process of claim 1 , wherein step c) is performed for 20 to 60 hours.

7. The process of claim 6 , wherein step c) is performed for about 24-48 hours.

8. The process of claim 1 , wherein the total time to perform step b) and step c) is 72 to 150 hours.

9. The process of claim 1 , wherein step b) is performed at a temperature of about 55° C.

10. The process of claim 1 , wherein step b) is performed at a temperature of about 60° C.

11. The process of claim 1 , wherein step b) is performed at a temperature of about 65° C.

12. The process of claim 1 , wherein the dry matter content of said corn fiber, corn stover or wheat straw in step b) is about 14-25%.

13. The process of claim 1 , wherein the filamentous fungal strain is from the genus Penicillium or Talaromyces.

14. The process of claim 13 , wherein the filamentous fungal strain is Talaromyces emersonii.

15. The process of claim 1 , wherein step c) is performed under oxygen-limited conditions.

16. The process of claim 1 , wherein the biofuel is ethanol or butanol.

17. The process of claim 16 , wherein the biofuel is ethanol.

18. The process of claim 1 , wherein step b) results in a release of 90% of available sugar in the corn fiber, corn stover or wheat straw.

Assignees

• Dsm Ip Assets Bv

Inventors

• Smits Johannes Petrus
• Gierveld Elisabeth Maria
• Van Der Hor Fop

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12R2001/645

  Fungi {; Processes using fungi} · CPC title