Production of low-alcohol or alcohol-free beer with Pichia kluyveri yeast strains
US-11162059-B2 · Nov 2, 2021 · US
US12467022B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12467022-B2 |
| Application number | US-201817265604-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 10, 2018 |
| Priority date | Sep 10, 2018 |
| Publication date | Nov 11, 2025 |
| Grant date | Nov 11, 2025 |
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The present invention discloses a method for preparing beer having an ethanol content of 0-1.0 vol. %, comprising providing a medium having an ethanol content of 0-20 vol. %, which medium comprises restricted-fermentation beer; subjecting said medium to a distillation step, thereby reducing the quantity of one or more aldehydes selected from the group consisting of 2-methyl-propanal, 2-methylbutanal, 3-methylbutanal, 3-methylthiopropionaldehyde, phenylacetaldehyde, hexanal, trans-2-nonenal, benzaldehyde and furfural in the medium, and thereby also reducing the ethanol content, if present, to a content in the range of 0-1.0 vol. %. The invention furthermore discloses beer obtained with the said process.
Opening claim text (preview).
The invention claimed is: 1 . A method for preparing beer having an ethanol content of 0-0.2 vol. %, comprising subjecting a medium comprising restricted-fermentation beer having an ethanol content from 1.0 vol % or less and regular beer having an ethanol content from 2.0 to 15 vol %, wherein the restricted fermentation beer is present in a ratio with the regular beer of 5:95 to 50:50, to a distillation step, thereby reducing the quantity of one or more aldehydes selected from the group consisting of 2-methylpropanal, 2-methylbutanal, 3-methylbutanal, 3-methylthiopropionaldehyde, phenylacetaldehyde, hexanal, trans-2-nonenal, benzaldehyde and furfural in the medium, and thereby also reducing the ethanol content to a content in the range of 0-0.2 vol. %. 2 . The method according to claim 1 , wherein the distillation is performed at a temperature of 10-100° C. 3 . The method according to claim 1 , wherein the distillation is performed at a pressure of 0.01-500 mbar. 4 . The method according to claim 1 , wherein the regular beer has an ethanol content from 3.5-9 1 15 vol. %. 5 . The method according to claim 1 , wherein the quantity of 2-methylpropanal is reduced to less than 18 μg/l; and/or the quantity of 2-methylbutanal is reduced to less than 3.8 μg/l; and/or the quantity of 3-methylbutanal is reduced to less than 14 μg/l; and/or the quantity of 3-methylthiopropionaldehyde is reduced to less than 10 μg/l; and/or the quantity of phenylacetaldehyde is reduced to less than 20 μg/l. 6 . The method according to claim 1 , wherein the total of 2-methylpropanal, 2-methylbutanal, 3-methylbutanal, 3-methylthiopropionaldehyde, and phenylacetaldehyde is reduced to less than 50 μg/1. 7 . The method according to claim 1 , wherein the total of 2-methylpropanal, 2-methylbutanal, 3-methylbutanal, 3-methylthiopropionaldehyde, phenylacetaldehyde, hexanal, trans-2-nonenal, benzaldehyde and furfural is reduced to less than 80 μg/l. 8 . The method according to claim 1 , wherein the distillation is performed at a temperature of 20-65° C. 9 . The method according to claim 1 , wherein the distillation is performed at a temperature of 30-50° C. 10 . The method according to claim 1 , wherein the distillation is performed at a temperature of 40-46° C. 11 . The method according to claim 1 , wherein the distillation is performed at a pressure of 1-200 mbar. 12 . The method according to claim 1 , wherein the distillation is performed at a pressure of 50-150 mbar. 13 . The method according to claim 1 , wherein the distillation is performed at a pressure of 80-110 mbar. 14 . The method according to claim 1 , wherein the regular beer has an ethanol content from 2.5-10 vol. %. 15 . The method according to claim 1 , wherein the quantity of 2-methylpropanal is reduced to less than 10 μg/l; and/or the quantity of 2-methylbutanal is reduced to 0.2-2.5 μg/l; and/or the quantity of 3-methylbutanal is reduced to 1-10 μg/l; and/or the quantity of 3-methylthiopropionaldehyde is reduced to 2-8 μg/l; and/or the quantity of phenylacetaldehyde is reduced to 1-8 μg/l. 16 . The method according to claim 1 , wherein the quantity of 2-methylpropanal is reduced to 2-10 μg/l. 17 . The method according to claim 1 , wherein the regular beer comprises lager beer. 18 . The method according to claim 1 , wherein the regular beer comprises pilsner. 19 . The method according to claim 1 , wherein the restricted-fermentation beer is a fermented beer obtained by restricted ethanol fermentation of wort characterized by a temperature below 7° C. for a period of 8-72 hours (“cold contact fermented beer”); and/or a fermentation time of less than 2 hours, which fermentation is stopped quickly by rapid cooling to −0.5-1° C., optionally followed by subsequent pasteurization (“arrested fermentation beer”); and/or fermentation by a yeast strain which produces less than 0.2 g ethanol per gram fermentable sugar in the wort under the applied fermentation conditions, wherein suitable strains comprise Crabtree negative strains, and the quantity of ethanol produced under varying fermentation conditions can be determined by routine experiments (“yeast-restricted beer”); and/or fermentation using a first, ethanol-producing yeast strain, in the presence of a sufficient quantity of a second yeast strain which consumes ethanol to consume substantially all ethanol produced by the first yeast strain; and/or wort having a content of fermentable sugars such that max 1.0 vol. % of alcohol is produced after completion of its fermentation, wherein the wort generally has a content of fermentable sugars of less than 17.5 g/l (“sugar-deprived wort beer”). 20 . The method according to claim 19 , wherein the fermentation to obtain a cold contact fermented beer is characterized by a temperature of −1-4° C. for a period of 8-72 hours; and/or the fermentation to obtain a yeast restricted beer is characterized by use of a yeast strain which produces less than 0.1 g ethanol per gram fermentable sugar under the applied fermentation conditions; and/or the yeast strain which consumes ethanol is Saccharomyces rouxii ; and/or the fermentation to obtain a sugar-deprived wort beer is characterized in that the wort has a content of fermentable sugars of less than 12 g/l. 21 . The method according to claim 19 wherein the fermentation to obtain a cold contact fermented beer is characterized by a temperature of −0.5-2.5° C. for a period of 12-48 hrs; and/or; the fermentation to obtain a sugar-deprived wort beer is characterized in that the wort has a content of fermentable sugars of less than 8 g/l. 22 . The method according to claim 1 , wherein the regular beer comprises ethyl-2-methylpentanoate.
Yeasts (processes for seeding C12C11/02) · CPC title
Fermentation processes for beer (preparation of wine C12G1/00) · CPC title
by evaporating · CPC title
Biofuels, e.g. bio-diesel · CPC title
Beer with low alcohol content (methods for reducing the alcohol content after fermentation C12H3/00) · CPC title
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