Polynucleotide sequences and proteins encoded thereby useful for modifying plant characteristics

Patent metadata

Field	Value
Publication number	US-12448627-B2
Application number	US-202017115424-A
Country	US
Kind code	B2
Filing date	Dec 8, 2020
Priority date	Jul 5, 2007
Publication date	Oct 21, 2025
Grant date	Oct 21, 2025

Abstract

The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides. The present invention further relates to the uses of these nucleic acid molecules and polypeptides. For example, the nucleic acid molecules and polypeptides could be used in making enzymes or used to make plants, plant cells, plant materials or seeds of a plant having such modulated growth or phenotype characteristics that are altered with respect to wild type plants grown under similar conditions.

First claim

What is claimed is:

1. A method of producing a transgenic switchgrass plant, said method comprising
introducing into a switchgrass plant cell a nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide, wherein:
(i) the polypeptide comprises the amino acid sequence of SEQ ID NO:76; or
(ii) the nucleotide sequence comprises the polynucleotide sequence of SEQ ID NO:75;

 wherein the nucleic acid molecule is operably linked to a heterologous promoter.

2. The method of claim 1 , wherein said promoter is selected from the group consisting of YP0092, PT0676, PT0708, PT0613, PT0678, PT0688, PT0837, a napin promoter, an Arcelin-5 promoter, a phaseolin gene promoter, a soybean trypsin inhibitor promoter, an ACP promoter, a stearoyl-ACP desaturase gene promoter, a soybean a′ subunit of 13-conglycinin promoter, an oleosin promoter, a 15 kD zein promoter, a 16 kD zein promoter, a 19 kD zein promoter, a 22 kD zein promoter, a 27 kD zein promoter, an Osgt-1 promoter, a beta-amylase gene promoter, a barley hordein gene promoter, p326, YP0144, YP0190, p13879, YP0050, p32449, 21876, YP0158, YP0214, YP0380, PT0848, PT0633, a cauliflower mosaic virus 35S promoter, a mannopine synthase promoter, a 1′ or 2′ promoter derived from T-DNA of Agrobacterium tumefaciens , a figwort mosaic virus 34S promoter, a rice actin promoter, a maize ubiquitin-1 promoter, a RbcS promoter from eastern larch ( Larix laricina ), a pine cab6 promoter, a Cab-1 gene promoter from wheat, a CAB-1 promoter from spinach, a cab1 R promoter from rice, a pyruvate orthophosphate dikinase (PPDK) promoter from corn, a tobacco Lhcb1*2 promoter, an Arabidopsis thaliana SUC2 sucrose-H+ symporter promoter, and a thylakoid membrane protein promoter.

3. A method for producing a transgenic switchgrass plant, comprising:
(a) transforming a switchgrass plant with a nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide, wherein:
(i) the polypeptide comprises the amino acid sequence of SEQ ID NO:76; or
(ii) the nucleotide sequence comprises the polynucleotide sequence of SEQ ID NO:75;

wherein the nucleic acid molecule is operably linked to a heterologous promoter.

4. A method for producing a transgenic switchgrass the method steps comprising:
(a) inserting a nucleic acid molecule into an expression vector wherein the nucleic acid molecule comprises a nucleotide sequence encoding a polypeptide, wherein:
(i) the polypeptide comprises the amino acid sequence of SEQ ID NO:76; or
(ii) the nucleotide sequence comprises the polynucleotide sequence of SEQ ID NO:75; wherein the nucleic acid molecule is operably linked to a heterologous promoter;

(b) introducing the vector into a switchgrass plant or a cell of a switchgrass plant to overexpress the encoded polypeptide of the nucleotide sequence; and
(c) selecting the transgenic switchgrass plant for overexpressing the encoded polypeptide.

5. The method of claim 1 , wherein the nucleotide sequence comprises the polynucleotide sequence of SEQ ID NO:75.

6. The method of claim 1 , wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:76.

7. The method of claim 3 , wherein the nucleotide sequence comprises the polynucleotide sequence of SEQ ID NO:75.

8. The method of claim 3 , wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:76.

9. The method of claim 4 , wherein the nucleotide sequence comprises the polynucleotide sequence of SEQ ID NO:75.

10. The method of claim 4 , wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:76.

Assignees

• Ceres Inc

Inventors

• Brover Vyacheslav
• Swaller Timothy J
• Feldmann Kenneth A
• Troukhan Maxim

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• A23L27/10

  Natural spices, flavouring agents or condiments; Extracts thereof · CPC title

• A23L19/00

  Products from fruits or vegetables; Preparation or treatment thereof (marmalades, jams, jellies or the like A23L21/10; treating harvested fruit or vegetables in bulk A23N) · CPC title

• A23L7/10

  Cereal-derived products · CPC title