Polynucleotide sequences and proteins encoded thereby useful for modifying plant characteristics

Patent metadata

Field	Value
Publication number	US-10150971-B2
Application number	US-201615062829-A
Country	US
Kind code	B2
Filing date	Mar 7, 2016
Priority date	Jul 5, 2007
Publication date	Dec 11, 2018
Grant date	Dec 11, 2018

Abstract

The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides. The present invention further relates to the uses of these nucleic acid molecules and polypeptides. For example, the nucleic acid molecules and polypeptides could be used in making enzymes or used to make plants, plant cells, plant materials or seeds of a plant having such modulated growth or phenotype characteristics that are altered with respect to wild type plants grown under similar conditions.

First claim

What is claimed is:

1. A nucleic acid molecule comprising:
(a) a nucleotide sequence encoding an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO:26351;
(b) a nucleotide sequence that is at least 95% identical to the nucleotide sequence of SEQ ID NO:26350;
(c) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:26351; or
(d) a nucleotide sequence comprising the nucleotide sequence of SEQ ID NO:26350;
wherein the nucleotide sequence encodes a polypeptide that comprises a zf-Dof domain and increases biomass of a plant when expressed in the plant; and
wherein the nucleic acid molecule is operably linked to a heterologous regulatory region.

2. A vector, comprising the nucleic acid molecule of claim 1 .

3. A method of increasing biomass, said method comprising introducing into a plant cell the nucleic acid molecule according to claim 1 , wherein a plant produced from said plant cell is selected for having increased biomass as compared to a control plant that does not comprise said nucleic acid molecule.

4. The method of claim 1 , wherein said regulatory region is a promoter selected from the group consisting of YP0092, PT0676, PT0708, PT0613, PT0678, PT0688, PT0837, the napin promoter, the Arcelin-5 promoter, the phaseolin gene promoter, the soybean trypsin inhibitor promoter, the ACP promoter, the stearoyl-ACP desaturase gene, the soybean a′ subunit of 13-conglycinin promoter, the oleosin promoter, the 15 kD zein promoter, the 16 kD zein promoter, the 19 kD zein promoter, the 22 kD zein promoter, the 27 kD zein promoter, the Osgt-1 promoter, the beta-amylase gene promoter, the barley hordein gene promoter, p326, YP0144, YP0190, p138′79, YP0050, p32449, 21876, YP0158, YP0214, YP0380, PT0848, PT0633, the cauliflower mosaic virus 35S promoter, the mannopine synthase promoter, the 1′ or 2′ promoters derived from T-DNA of Agrobacterium tumefaciens , the figwort mosaic virus 34S promoter, actin promoters such as the rice actin promoter, ubiquitin promoters such as the maize ubiquitin-1 promoter, ribulose-1,5-bisphosphate carboxylase promoters such as the RbcS promoter from eastern larch ( Larix laricina ), the pine cab6promoter, the Cab-1 gene promoter from wheat, the CAB-1 promoter from spinach, the cab1 R promoter from rice, the pyruvate orthophosphate dikinase (PPDK) promoter from corn, the tobacco Lhcb 1*2 promoter, the Arabidopsis thaliana SUC2 sucrose-H+symporter promoter, and thylakoid membrane protein promoters from spinach (psaD, psaF, psaE, PC, FNR, atpC, atpD, cab, rbcS, PT0535, PT0668, PT0886, PR0924, and PT0585).

5. A plant cell comprising the nucleic acid molecule of claim 1 , wherein a plant produced from said plant cell is selected for having increased biomass as compared to a control plant that does not comprise said nucleic acid.

6. A transgenic plant comprising the plant cell of claim 5 .

7. Progeny of the transgenic plant of claim 6 , wherein said progeny comprises said nucleic acid molecule and has increased biomass as compared to a control plant that does not comprise said nucleic acid.

8. Seed from the transgenic plant according to claim 6 wherein said seed comprises said nucleic acid molecule.

9. Vegetative tissue from the transgenic plant according to claim 6 .

10. A food product comprising vegetative tissue from the transgenic plant according to claim 6 .

11. A feed product comprising vegetative tissue from the transgenic plant according to claim 6 .

12. A method for increasing biomass of a transformed plant, comprising:
(a) transforming a plant with the nucleic acid molecule according to claim 1 ; and
(b) expressing said nucleotide sequence in said transformed plant, whereby said transformed plant is selected for having increased biomass as compared to a plant that has not been transformed with said nucleic acid molecule.

13. A method for increasing biomass of a plant, said method comprising increasing the level of expression in said plant of the nucleic acid molecule according to claim 1 .

14. A transgenic plant having increased biomass as compared to a wild-type plant of the same species, wherein the transgenic plant comprises a recombinant polynucleotide comprising the nucleic acid molecule according to claim 1 , and wherein the polypeptide encoded by said nucleotide sequence confers to the transgenic plant increased biomass as compared to a wild-type plant of the same species.

15. A method for producing a transgenic plant having increased biomass as compared to a wild-type plant of the same species, the method steps comprising: (a) inserting the nucleic acid molecule according to claim 1 into an expression vector; (b) introducing the vector into a plant or a cell of a plant to overexpress the encoded polypeptide of the nucleotide sequence; and (c) selecting a transgenic plant for increased biomass as compared to the wild-type plant of the same species.

Assignees

• Ceres Inc

Inventors

• Brover Vyacheslav
• Swaller Timothy J
• Feldmann Kenneth A
• Troukhan Maxim

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• A23L27/10

  Natural spices, flavouring agents or condiments; Extracts thereof · CPC title

• C12Q2600/158

  Expression markers · CPC title