Ion accumulation control for analytical instrument

Patent metadata

Field	Value
Publication number	US-12437984-B2
Application number	US-202318095860-A
Country	US
Kind code	B2
Filing date	Jan 11, 2023
Priority date	Jan 10, 2022
Publication date	Oct 7, 2025
Grant date	Oct 7, 2025

Abstract

A method of operating an instrument which comprises a first and second ion stores, comprising determining whether a target accumulation time for the second ion store is greater than a threshold accumulation time. When the target accumulation time is less than the threshold accumulation time, ions are accumulated within the second ion store using an accumulation time that is based on the target accumulation time. When the target accumulation time is greater than the threshold accumulation time, ions are accumulated within the first ion store using a first accumulation time that is based on a difference between the target accumulation time and the threshold accumulation time, the ions accumulated in the first ion store are passed to the second ion store, and further ions are accumulated within the second ion store using a second accumulation time that is based on the threshold accumulation time.

First claim

The invention claimed is:

1. A method of operating an analytical instrument that comprises a first ion store and a second ion store arranged downstream of the first ion store, the method comprising:
determining whether a target accumulation time for the second ion store is greater than a threshold accumulation time;
when it is determined that the target accumulation time is less than the threshold accumulation time: accumulating ions within the second ion store using an accumulation time based on the target accumulation time; and
when it is determined that the target accumulation time is greater than the threshold accumulation time: accumulating ions within the first ion store using a first accumulation time based on a difference between the target accumulation time and the threshold accumulation time, passing the ions accumulated in the first ion store to the second ion store, and accumulating further ions within the second ion store using a second accumulation time based on the threshold accumulation time.

2. The method of claim 1 , wherein the instrument comprises at least one first gate configured to control an accumulation time of ions in the first ion store, and at least one second gate configured to control an accumulation time of ions in the second ion store, wherein a response time of the at least one second gate is faster than a response time of the at least one first gate.

3. The method of claim 1 , wherein the instrument comprises an ion source and one or more ion optical devices arranged between the ion source and the second ion store, wherein the one or more ion optical devices are configured to transmit ions from the ion source to the second ion store, and wherein the first ion store is arranged within the one or more ion optical devices.

4. The method of claim 3 , wherein the first ion store is formed in a transfer ion guide of the one or more ion optical devices.

5. The method of claim 1 , wherein the instrument includes a first mass filter arranged upstream of the second ion store, and wherein the first ion store is arranged upstream of the first mass filter.

6. The method of claim 5 , wherein the instrument includes a second mass filter arranged upstream of the first mass filter, wherein a resolution of the second mass filter is less than a resolution of the first mass filter, and wherein the first ion store is arranged between the first mass filter and the second mass filter.

7. The method of claim 5 , further comprising the first mass filter filtering ions according to their mass to charge ratio, wherein the first mass filter filters ions using an isolation window having a width>about 2 Da.

8. The method of claim 1 , wherein the instrument comprises a mass analyser arranged downstream of the second ion store, and wherein the method comprises passing ions accumulated in the second ion store to the mass analyser, and analysing the ions using the mass analyser.

9. The method of claim 8 , wherein the mass analyser analysing the ions produces a time-varying transient signal, and wherein the method further comprises producing a mass spectrum from the time-varying transient signal using a phase-constrained spectrum deconvolution method (@SDM).

10. The method of claim 9 , wherein the time-varying transient signal has a duration<50 ms.

11. The method of claim 1 , wherein the instrument is operated in a cyclical manner, and wherein the threshold accumulation time is based on a difference between a total cycle time for the instrument and a time per cycle in which the instrument is operated in a mode in which ions are other than accumulated in the second ion store.

12. The method of claim 11 , wherein the time per cycle in which the instrument is operated in the mode in which ions are other than accumulated in the second ion store comprises a time per cycle in which the second ion store is operated in a non-accumulating mode of operation while ions accumulated in the second ion store are processed and/or passed to a mass analyser for analysis.

13. The method of claim 11 , further comprising operating the instrument with a repetition rate>20 Hz, >40 Hz, >60 Hz, or >80 Hz.

14. The method of claim 1 , wherein accumulating ions within the second ion store using an accumulation time based on the target accumulation time comprises:
operating the first ion store in a transmissive mode of operation during the accumulation time, such that ions pass through the first ion store during the accumulation time, without being accumulated within the first ion store; and
operating the second ion store in an accumulation mode during the accumulation time, such that ions are accumulated within the second ion store during the accumulation time.

15. The method of claim 1 , wherein accumulating ions within the first ion store using the first accumulation time comprises:
operating the first ion store in an accumulation mode during the first accumulation time, such that ions are accumulated within the first ion store during the first accumulation time.

16. The method of claim 1 , wherein passing the ions accumulated in the first ion store to the second ion store comprises:
operating the first ion store in transmissive mode such that ions accumulated in the first ion store are passed to the second ion store; and
operating the second ion store in an accumulation mode, such that ions passed to the second ion store from the first ion store are accumulated within the second ion store.

17. The method of claim 1 , wherein accumulating further ions within the second ion store using the second accumulation time comprises:
operating the first ion store in a transmissive mode during the second accumulation time, such that ions pass through the first ion store during the second accumulation time, without being accumulated within the first ion store; and
operating the second ion store in an accumulation mode during the second accumulation time, such that ions are accumulated within the second ion store during the second accumulation time.

18. The method of claim 1 , wherein when it is determined that the target accumulation time is equal to the threshold accumulation time, the method comprises accumulating ions within the second ion store using an accumulation time based on the target accumulation time.

19. A non-transitory computer readable storage medium storing computer software code which when executed on a processor causes an analytical instrument that comprises a first ion store and a second ion store arranged downstream of the first ion store to perform the steps of:
determining whether a target accumulation time for the second ion store is greater than a threshold accumulation time;
when it is determined that the target accumulation time is less than the threshold accumulation time: accumulating ions within the second ion store using an accumulation time based on the target accumulation time; and
when it is determined that the target accumulation time is greater than the threshold accumulation time: accumulating ions within the first ion store using a first accumulation time based on a difference between the target accumulation time and the threshold accumulation time, passing the ions accumulated in the first ion store to the second ion store, and accumulating further ions within the second ion store using a second accumulation time based on the threshold accumulation time.

20. An analytical instrument, such as a mass spectrometer, comprising:
a first ion store;
a second ion store, wherein the second ion store is arranged downstre

Assignees

• Thermo Fisher Scient Bremen Gmbh

Inventors

• Stewart Hamish
• Makarov Alexander
• Ayzikov Konstantin
• Grinfeld Dmitry
• Fort Kyle
• Arrey Tabiwang
• Biel Matthias

Classifications

• H01J49/4265 Primary

  Controlling the number of trapped ions; preventing space charge effects · CPC title

• H01J49/062

  Ion guides (linear ion traps performing mass selection H01J49/4225, mass filters H01J49/421) · CPC title

• H01J49/425

  with a logarithmic radial electric potential, e.g. orbitraps · CPC title

• H01J49/0045

  characterised by the fragmentation or other specific reaction · CPC title

• H01J49/0036

  Step by step routines describing the handling of the data generated during a measurement · CPC title