Systems and methods for specimen processing and storage

Patent metadata

Field	Value
Publication number	US-12437398-B2
Application number	US-202117997281-A
Country	US
Kind code	B2
Filing date	Apr 29, 2021
Priority date	Apr 29, 2020
Publication date	Oct 7, 2025
Grant date	Oct 7, 2025

Abstract

A method including receiving a specimen comprising a carrier, a first target species, and a first component and storing at least a portion of the carrier and the first target species in a storage media by self-driven filtering of the specimen in the storage media, wherein the storage media comprises porous superabsorbent polymer (PSAP) beads. The PSAP beads provide for fast and self-driven microfiltration of biofluid samples. The treatment effectively separates small analytical targets (e.g., glucose, catalase, and bacteriophage) and large undesired components (e.g., bacteria and blood cells) in the biofluids by capturing the former inside and excluding the latter outside the PSAP beads. The treatment can reduce sample volume, self-aliquot the liquid sample, avoid microbial contamination, separate plasma from blood cells, stabilize target species inside the beads, and enable long-term storage at room temperature.

First claim

What is claimed is:

1. A method comprising:
Introducing a specimen comprising a carrier and components to a storage media; and
Releasing at least a portion of a first target from the storage media;
Wherein:
At least a portion of the components of the specimen comprise a first target;
The storage media is configured to capture at least a portion of the first target; and
The storage media is tunable, comprises a superabsorbent polymer, and has a selective absorption profile configured:
To capture components having a size less than a capture size of approximately 0.5 μm; and
To exclude components having a size greater than a non-capture size of approximately 1.0 μm.

2. The method of claim 1 , wherein at least one of:
the introducing comprises storing at least a portion of the carrier and the first target in the storage media;
the storage media has a swelling capacity profile configured to absorb and capture the first target and the carrier;
the first target has a size less than the capture size; or
the introducing comprises self-driven filtering of the specimen in the storage media resulting in the absorption and capture of the first target and the carrier in the storage media.

3. The method of claim 1 further comprising:
removing unstored specimen from proximity of the storage media.

4. The method of claim 1 , wherein at least one of:
the specimen comprises a biofluid;
the storage media comprises porous superabsorbent polymer beads;
the specimen comprises a biofluid, the storage media comprises porous superabsorbent polymer beads, and the porous superabsorbent polymer beads have a porous structure for the selective absorption of the first target in the biofluid;
the specimen comprises a biofluid, the storage media comprises porous superabsorbent polymer beads, and the porous superabsorbent polymer beads have a swelling capacity profile to capture the biofluid together with first target inside the beads of the porous superabsorbent polymer beads;
the releasing comprises adding water to the storage media that comprises porous superabsorbent polymer beads; or
the releasing comprises adding water to the storage media that comprises porous superabsorbent polymer beads and sonicating the water/porous superabsorbent polymer beads mixture for a time period sufficient to release at least a portion of the first target from the porous superabsorbent polymer beads.

5. The method of claim 1 , wherein at least one of:
the components are selected from a group consisting of glucose, catalase, bacteriophage, bacteria, blood cells, and combinations thereof; and wherein or
the first target is selected from the group consisting of glucose, catalase, and bacteriophage.

6. The method of claim 1 , wherein:
the first target is a first target species;
introducing comprises storing at least a portion of the carrier and the first target species in the storage media by self-driven filtering of the specimen in the storage media;
the storage media comprises porous superabsorbent polymer beads;
the method further comprises removing unstored specimen from proximity of the porous superabsorbent polymer beads;
the releasing comprises releasing at least a portion of the first target species from the porous superabsorbent polymer beads;
the porous superabsorbent polymer beads have a swelling capacity profile configured to capture and absorb the first target species and the carrier in the porous superabsorbent polymer beads.

7. The method of claim 6 , wherein the releasing comprises:
adding water to the porous superabsorbent polymer beads; and
sonicating the water/porous superabsorbent polymer beads mixture for a time period sufficient to release at least a portion of the first target species from the porous superabsorbent polymer beads.

8. The method of claim 7 , wherein:
the water is deionized water; and
sonicating is ultrasonicating.

9. The method of claim 1 , wherein:
the specimen is a sample;
the first target is a first analytical target;
the storage media comprises porous superabsorbent polymer beads;
the introducing comprises self-aliquoting the sample by introducing the sample to the porous superabsorbent polymer beads that capture at least a portion of the first analytical target; and
the porous superabsorbent polymer beads are tuned in order to enable storage at room temperature of the stored first analytical target in the porous superabsorbent polymer beads.

10. The method of claim 9 , wherein the porous superabsorbent polymer beads are further tuned to avoid microbial contamination.

11. The method of claim 9 , wherein the porous superabsorbent polymer beads are further tuned to;
present a capture size porosity configured to capture the first analytical target having a size less than or equal to the capture size porosity; and
exclude from capture components of the sample having a size larger than the capture size porosity.

12. The method of claim 11 , wherein the porous superabsorbent polymer beads are further tuned to separate plasma from blood cells.

13. The method of claim 9 , wherein:
a stored first analytical target is analyzed after storage; and
a length of storage at room temperature is at least 1 day without degradation of the first analytical target to a point that it cannot be analyzed.

14. The method of claim 13 , wherein the length of storage is at least 3 days.

15. The method of claim 13 , wherein the length of storage is at least 7 days.

16. The method of claim 15 , further comprising:
analyzing the stored first analytical target for enzymatic activities.

17. The method of claim 1 , wherein:
the specimen is a sample;
the first target is a first analytical target;
the storage media comprises porous superabsorbent polymer beads;
the introducing comprises self-aliquoting the sample by introducing the sample to the porous superabsorbent polymer beads that capture at least a portion of the first analytical target; and
the porous superabsorbent polymer beads are tuned in order to enable testing of a stored first analytical target at a date/time subsequent to the introducing.

18. The method of claim 17 , wherein the testing is testing for enzymatic activities of the first analytical target.

19. The method of claim 17 , wherein the porous superabsorbent polymer beads have a polyethylene glycol (PEG) content of at least 2.5 wt %.

20. The method of claim 17 , wherein the porous superabsorbent polymer beads have a PEG content of between 7.5 wt % and 20 wt %.

21. The method of claim 17 , wherein the porous superabsorbent polymer beads have a PEG content of between 10 wt % and 15 wt %.

22. The method of claim 17 , wherein the porous superabsorbent polymer beads are tuned to have an average pore diameter of between 0.15 to 0.3 m.

23. The method of claim 9 , wherein at least one of:
the porous superabsorbent polymer beads have a PEG content of between 2.5 wt % and 20 wt %; or
the porous superabsorbent polymer beads are tuned to have an average pore diameter of between 0.15 to 0.3 μm.

24. The method of claim 17 , wherein the porous superabsorbent polymer beads are further tuned to avoid microbial contamination.

25. The method of claim 17 , wherein the porous superabsorbent polymer beads are further tuned to enable successful testing of the stored first analytical target 1 day after introducing the sample to the porous superabsorbent polymer beads.

Assignees

• Georgia Tech Res Inst

Inventors

• Xie Xing
• Chen Wensi

Classifications

• B01D61/147

  Microfiltration · CPC title

• G06T2207/30004

  Biomedical image processing · CPC title

• G01N15/1433

  using image recognition · CPC title

• B01J20/264

  derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers · CPC title

• B01J20/28059

  being less than 100 m2/g · CPC title