Systems and methods for multiple analyte detection

Patent metadata

Field	Value
Publication number	US-9714444-B2
Application number	US-201314093933-A
Country	US
Kind code	B2
Filing date	Dec 2, 2013
Priority date	Apr 26, 2005
Publication date	Jul 25, 2017
Grant date	Jul 25, 2017

Abstract

Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.

First claim

What is claimed is:

1. A system for distribution of a biological sample, the system comprising:
a substrate, wherein the substrate comprises a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber;
a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate; and
a sealing instrument comprising a roller provided with a plurality of sealing protrusions in the form of circumferential disks spaced from each other along a longitudinal axis of the roller such that each protrusion can align with a sample chamber and placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber,
wherein the substrate is constructed of detection-compatible and assay-compatible materials.

2. The system of claim 1 , wherein a sealing plate contacts the substrate during a reaction process in the sample chambers to seal each sample chamber.

3. The system of claim 2 , further comprising a thermal block, wherein the thermal block comprises the sealing plate.

4. The system of claim 1 , wherein the sealing instrument comprises one of a plate and a roller.

5. The system of claim 1 , wherein the substrate comprises a film layer and wherein a portion of the film layer fills the sample introduction channels and the venting channels upon contact of the sealing instrument with the substrate.

6. The system of claim 1 , wherein the sealing protrusions are configured to seal the sample introduction channels and venting channels for each sample chamber.

7. The system of claim 1 , further comprising an additional sealing instrument, wherein the plurality of sealing protrusions comprises a first plurality of sealing protrusions associated with the sealing instrument and a second plurality of the sealing protrusions associated with the additional sealing instrument, the first plurality and second plurality of sealing protrusions being configured to seal differing groups of sample chambers when the sealing instrument and the additional sealing instrument are respectively placed in contact with the substrate.

8. The system of claim 7 , wherein the first plurality of sealing protrusions is configured to seal the sample introduction channels for each sample chamber and the second plurality of sealing protrusions are configured to seal the venting channels for each sample chamber.

9. The system of claim 1 , wherein each sealing protrusion is configured to contact a respective sample introduction channel and venting channel for each sample chamber when the sealing instrument is placed in contact with the substrate.

10. The system of claim 1 , wherein the plurality of sealing protrusions are configured to respectively align with the plurality of sample chambers.

11. The system of claim 10 , wherein each sample chamber is configured to receive a respective sealing protrusion such that the sealing protrusions seal the sealing chambers substantially at an inner peripheral surface defining each sample chamber.

12. The system of claim 1 , wherein the sealing instrument further comprises a plurality of optical apertures configured to provide optical access to the sample chambers.

13. The system of claim 12 , wherein the sealing protrusions are configured to pierce the substrate and enter the introduction and venting channels for each sample chamber.

14. The system of claim 1 , wherein the sealing protrusions comprise one of pins, blades, and bumps.

15. The system of claim 1 , wherein the substrate further comprises a plurality of sample supply channels, each sample supply channel being in flow communication with a differing group of sample introduction channels and corresponding sample chambers.

16. The system of claim 15 , wherein the substrate further comprises a plurality of sample inlet ports, each sample inlet port being in flow communication with a differing sample supply channel.

17. The system of claim 1 , wherein the at least one venting mechanism comprises a plurality of venting mechanisms respectively corresponding to the plurality of sample chambers.

18. The system of claim 1 , wherein the substrate further comprises a venting chamber in flow communication with each venting channel.

19. The system of claim 1 , wherein the substrate further comprises a venting chamber for each sample chamber, the venting chambers being in flow communication with the sample chambers via the venting channels, wherein the at least one venting mechanism comprises a plurality of venting mechanisms each corresponding to a respective venting chamber.

20. The system of claim 1 , wherein the substrate further comprises a main venting channel in flow communication with the venting channels.

21. The system of claim 1 , wherein the substrate further comprises an additional film layer, the film layers being disposed on opposing surfaces of the base.

22. The system of claim 21 , wherein the additional film layer and the base together define the sample chambers, sample introduction channels, and venting channels.

23. The system of claim 1 , wherein the at least one venting mechanism is configured to substantially prevent sample from passing through the at least one venting mechanism and out of the substrate.

Assignees

• Applied Biosystems Llc

Inventors

• Yue Min
• Liu David M
• Roy Joy
• Chiang Yuh-Min
• Yang Joon Mo
• Lehto Dennis
• Vann Charles S
• Beard Nigel P
• Harding Ian A
• Van Camp John R
• Dromaretsky Alexander
• Ermakov Sergey V
• Oldham Mark F
• Shariati Maryam
• Ulmanella Umberto

Classifications

• B01L2200/16

  Reagents, handling or storing thereof · CPC title

• B01L2300/041

  Connecting closures to device or container · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• B01L2200/0684

  Venting, avoiding backpressure, avoid gas bubbles · CPC title

• B01L2300/0864

  comprising only one inlet and multiple receiving wells, e.g. for separation, splitting · CPC title