Enzyme extraction methods

Patent metadata

Field	Value
Publication number	US-12435452-B2
Application number	US-202117411590-A
Country	US
Kind code	B2
Filing date	Aug 25, 2021
Priority date	Feb 26, 2019
Publication date	Oct 7, 2025
Grant date	Oct 7, 2025

Abstract

Disclosed are methods of extracting an enzyme, comprising soaking a seed in an aqueous solution, homogenizing the seed to produce a homogenized suspension, and filtering the homogenized suspension to produce a crude extract, wherein the crude extract comprises urease and non-urease proteins.

First claim

What is claimed is:

1. A method of producing a powdered crude extract for biocementation, the method consisting of:
soaking a seed in an aqueous solution for between 12 and 24 hours to form a soaked seed, wherein the seed comprises jack bean, and wherein the aqueous solution consists of water;
subsequently homogenizing the soaked seed to produce a homogenized suspension with urease proteins and non-urease proteins;
filtering the homogenized suspension to produce a crude extract, wherein a purification fold of the crude extract is equal to 1; and
lyophilizing and/or drying the crude extract to produce the powdered crude extract, and wherein:
the powdered crude extract comprises urease proteins and non-urease proteins, and
the powdered crude extract is produced for a biocementation process for enzyme induced carbonate precipitation (EICP) treatment of soil.

2. The method of claim 1 , wherein the filtering comprises:
passing the homogenized suspension through a first filter to produce a solution; and
passing the solution through a second filter to produce the crude extract.

3. The method of claim 2 , wherein the second filter comprises glass wool.

4. The biocementation process of claim 1 , further comprising adding urea, a source of calcium ions, and an enzyme stabilizer to the powdered crude extract to produce a biocementation solution for the EICP treatment of the soil.

Assignees

• Univ Arizona State

Inventors

• Kavazanjian Edward
• Hamdan Nasser
• Tirkolaei Hamed Khodadadi
• Javadi Neda

Classifications

• C12Y305/01005

  Urease (3.5.1.5) · CPC title

• C12N9/96

  Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title

• C12N9/80

  acting on amide bonds in linear amides {(3.5.1)} · CPC title

• C09K17/42

  Inorganic compounds mixed with organic active ingredients, e.g. accelerators · CPC title

• D01G11/04 Primary

  Opening rags to obtain fibres for re-use (mechanical treatment of rags for paper-making D21B) · CPC title