De novo polynucleotide synthesis with substrate-bound polymerase

Patent metadata

Field	Value
Publication number	US-12428659-B2
Application number	US-202318306653-A
Country	US
Kind code	B2
Filing date	Apr 25, 2023
Priority date	May 28, 2020
Publication date	Sep 30, 2025
Grant date	Sep 30, 2025

Abstract

De novo polynucleotide synthesis is performed with a substrate-bound polymerase. The polymerase is attached to a solid substrate such as a microelectrode array. The polymerase adds nucleotides to growing polynucleotides strands that are also attached to the solid substrate. Spatial control of polymerase activity is achieved by changing the rate of nucleotide polymerization at selected locations on the surface of the solid substrate. The rate of polymerization is changed by inhibiting or promoting activity of the polymerase. In some implementations, activation of electrodes in the microelectrode array changes the rate of nucleotide polymerization. Nucleotides are added to the growing polynucleotide strands at areas where the polymerase is active. By varying the locations where the substrate-bound polymerase is active and the species of nucleotide added, a population of polynucleotides with different, arbitrary sequences is synthesized on the surface of the solid substrate.

First claim

The invention claimed is:

1. A solid substrate having polymerases and polynucleotides tethered to a surface, wherein:
the solid substrate comprises a microelectrode array,
the polymerases are tethered to the surface by a linker, or
the polynucleotides comprise 3′ blocking groups.

2. The solid substrate of claim 1 , wherein the solid substrate comprises a microelectrode array and the microelectrode array comprises a plurality of individually-addressable complementary metal-oxide-semiconductor (CMOS) circuits.

3. The solid substrate of claim 2 , wherein the microelectrode array has a microelectrode density of at least about 1024 microelectrodes/cm 2 .

4. The solid substrate of claim 1 , wherein the solid substrate comprises a microelectrode array and the polynucleotides comprise 3′ blocking groups, wherein the 3′ blocking groups are electrochemically cleavable by activity of an electrode of the microelectrode array.

5. The solid substrate of claim 1 , wherein the polymerases are terminal deoxynucleotide transferase (TdT) either wild-type or modified and either full-length or truncated.

6. The solid substrate of claim 1 , wherein the polymerases are tethered to the surface by a linker and the linker is selected from the group comprising poly(ethylene glycol) (PEG), alkyl chains, peptides, biotin-streptavidin linkages, and cross-linked enzyme aggregates (CELA).

7. The solid substrate of claim 1 , wherein the polynucleotides comprise 3′ blocking groups and the 3′ blocking groups are selected from the group comprising: esters, ethers, carbonitriles, phosphates, carbonates, carbamates, hydroxylamine, borates, nitrates, sugars, phosphoramide, phosphoramidates, phenylsulfenates, sulfates, sulfones, amino acids, 3′-O-amino, 3′-O-allyl, and a 3′-O-azidomethyl groups, O-phenoxyacetyl; O-methoxyacetyl; O-acetyl; O-(p-toluene)-sulfonate; O-phosphate; O-nitrate; O-[4-methoxy]-tetrahydrothiopyranyl; O-tetrahydrothiopyranyl; O-[5-methyl]-tetra-hydrofuranyl; O-[2-methyl, 4-methoxy]-tetrahydropyranyl; O-[5-methyl]-tetrahydropyranyl; and O-tetrahydrothiofuranyl.

8. The solid substrate of claim 1 , the solid substrate comprises a microelectrode array, the polymerases are tethered to the surface by a linker, and the polynucleotides comprise 3′ blocking groups.

9. A method of using polymerases tethered to a microelectrode array for solid-phase polynucleotide synthesis, the method comprising:
a. changing a rate of nucleotide polymerization that extends polynucleotides tethered to the surface of the microelectrode array at a selected location on the surface of the microelectrode array;
b. contacting the microelectrode array with a selected species of nucleotide;
c. washing the microelectrode array to remove free nucleotide;
d. iteratively repeating steps a-c, wherein the selected location or the selected species of nucleotide change at least once; and
e. separating the polynucleotides from the surface of the microelectrode array.

10. The method of claim 9 , wherein changing the rate of nucleotide polymerization comprises selectively deblocking polynucleotides tethered to the surface of the microelectrode array at the selected location.

11. The method of claim 10 , wherein the selectively deblocking comprises removing a blocking group from the 3′ end of the polynucleotides by a change in concentration of a chemical reagent, light, temperature, or pH.

12. The method of claim 9 , wherein changing the rate of nucleotide polymerization comprises selectively deblocking polynucleotides tethered to the surface of the microelectrode array at the selected location by cleaving electrochemically-cleavable blocking groups through activation of the microelectrode array.

13. The method of claim 9 , wherein changing the rate of nucleotide polymerization comprises changing an oxidation state of a cofactor of the polymerases through activation of the microelectrode array.

14. The method of claim 9 , wherein changing the rate of nucleotide polymerization comprises electrostatic sequestration of the polymerases or of the polynucleotides on the surface of the microelectrode array.

15. The method of claim 9 , wherein changing the rate of nucleotide polymerization comprises changing the pH of an aqueous solution in contact with the microelectrode array by a redox reaction initiated by activation of the microelectrode array at the selected location.

16. The method of claim 9 , further comprising tethering the polymerases to the surface of the microelectrode array by a hexahistidine (His-) tag, biotinylated peptides, amine-carboxylic acid equivalent bonds, amine-aldehyde bonds, an avidin sequence, a GST sequence, glutathione, a BiTag (AviTag) sequence, an S tag, a SNAP-tag, an antibody, an antibody domain, an antibody fragment, or an antigen.

17. A device for de novo synthesis of polynucleotides, the device comprising:
a solid substrate located within a reaction chamber and configured to have polymerases and polynucleotides tethered to a surface of the solid substrate;
means for changing a rate of nucleotide polymerization at a selected location on the surface of the solid substrate;
a fluid delivery pathway configured to contact the solid substrate with a selected nucleotide; and
control circuitry configured to change the selected location and activate the fluid delivery pathway.

18. The device of claim 17 , wherein the means for changing the rate of nucleotide polymerization comprises a microelectrode array, a targeted fluid deposition instrument, a light source, or a heater.

19. The device of claim 17 , wherein the means for changing the rate of nucleotide polymerization is configured to remove blocking groups from polynucleotides tethered to the surface of the solid substrate.

20. The device of claim 17 , wherein the means for changing the rate of nucleotide polymerization is configured to inhibit or promote activity of the polymerases.

Assignees

• Microsoft Technology Licensing Llc

Inventors

• Nguyen Bichlien
• Smith Jake
• Strauss Karin

Classifications

• B01J2219/00722

  Nucleotides · CPC title

• B01J2219/00596

  Solid-phase processes · CPC title

• B01J19/0046

  Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00) · CPC title

• C12P19/34 Primary

  Polynucleotides, e.g. nucleic acids, oligoribonucleotides · CPC title

• C12Q1/6844 Primary

  Nucleic acid amplification reactions · CPC title