Phosphoenolpyruvate carboxylase variant and method for producing 5à ¢â,¬â,,¢-inosinic acid using same

Patent metadata

Field	Value
Publication number	US-12410455-B2
Application number	US-202318879948-A
Country	US
Kind code	B2
Filing date	Aug 31, 2023
Priority date	Apr 6, 2023
Publication date	Sep 9, 2025
Grant date	Sep 9, 2025

Abstract

The present invention relates to a novel phosphoenolpyruvate carboxylase variant and a method of producing 5′-inosinic acid using the same. The phosphoenolpyruvate carboxylase variant is obtained by substituting one or more amino acids in the amino acid sequence constituting phosphoenolpyruvate carboxylase to change the activity of the protein, and a recombinant microorganism comprising the phosphoenolpyruvate carboxylase variant is capable of efficiently producing 5′-inosinic acid.

First claim

The invention claimed is:

1. A phosphoenolpyruvate carboxylase variant in which one or more of the amino acids at positions 472 and 673 in the amino acid sequence of SEQ ID NO: 4 is/are substituted with other amino acid(s).

2. The phosphoenolpyruvate carboxylase variant of claim 1 , which consists of the amino acid sequence of SEQ ID NO: 2 in which proline at position 472 in the amino acid sequence of SEQ ID NO: 4 is substituted with serine and glycine at position 673 is substituted with aspartic acid.

3. A polynucleotide encoding the variant of claim 1 .

4. A transformant host cell comprising the variant of claim 1 or the polynucleotide of claim 3 .

5. The transformant host cell of claim 4 , which is a Corynebacterium sp. microorganism.

6. The transformant host cell of claim 4 , which has ability to produce 5′-inosinic acid.

7. A method for producing 5′-inosinic acid, comprising steps of: culturing the transformant host cell of claim 4 in a medium; and recovering 5′-inosinic acid from the transformant host cell or the medium in which the transformant host cell has been cultured.

8. A transformant host cell comprising the polynucleotide of claim 3 .

Assignees

• Daesang Corp

Inventors

• Lee Sun Hee
• Choi Tae Yeol
• Kim Hyun Ho
• Kim Dong Hyun
• Kim Hyun Sook
• Shin Jong Hwan

Classifications

• C12P19/40

  having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides · CPC title

• C12Y401/01031

  Phosphoenolpyruvate carboxylase (4.1.1.31) · CPC title

• C12N9/88

  Lyases (4.) · CPC title

• C12R2001/15

  Corynebacterium · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title