Use of enzyme that cleaves both alpha- and beta- 1,4-glycosidic bonds

Patent metadata

Field	Value
Publication number	US-12410419-B2
Application number	US-202017595616-A
Country	US
Kind code	B2
Filing date	May 21, 2020
Priority date	May 21, 2019
Publication date	Sep 9, 2025
Grant date	Sep 9, 2025

Abstract

The present invention relates to use of an enzyme that cleaves both alpha- and beta-1,4-glycosidic bonds and, more particularly, the present invention provides α-L-fucosidase which has α-1,4-glucosidase and β-1,4-galactosidase activities and thus is capable of producing glucose and/or galactose, which are monosaccharides, from non-fucosylated saccharides. The enzyme has transglycosylation activity and thus produces maltooligosaccharides having a higher degree of polymerization than maltooligosaccharides used as a substrate, thereby being applicable to the development of high-value-added new materials in the food industry.

First claim

What is claimed is:

1. A composition for producing monosaccharides or maltooligosaccharides, comprising:
α-L-fucosidase comprising an amino acid sequence of SEQ ID NO: 1; and
one or more substrates selected from the group consisting of lactose and maltooligosaccharides having a degree of polymerization of 2 or higher,
wherein the α-L-fucosidase is obtained from a host cell transformed with a recombinant vector comprising a gene encoding the α-L-fucosidase.

2. The composition of claim 1 , wherein the α-L-fucosidase has α-1,4 glucosidase and β-1,4 galactosidase activities and transglycosylation activity.

3. The composition of claim 1 , wherein the gene encoding the α-L-fucosidase is derived from Vibrio sp. strain EJY3 having accession number KCTC11976BP.

4. The composition of claim 1 , wherein the gene comprises a base sequence of SEQ ID NO: 2.

5. The composition of claim 1 , wherein the monosaccharides comprise glucose or galactose.

6. The composition of claim 1 , wherein maltooligosaccharides having a degree of polymerization of 2 to 4 are used as the substrate.

7. A method for producing monosaccharides or maltooligosaccharides, comprising:
reacting one or more substrates selected from the group consisting of lactose and maltooligosaccharides having a degree of polymerization of 2 or higher with α-L-fucosidase comprising an amino acid sequence of SEQ ID NO: 1; and
collecting monosaccharides and maltooligosaccharides from a reaction product, wherein the maltooligosaccharides have a higher degree of polymerization than that of maltooligosaccharides used as the substrate.

8. The method of claim 7 , wherein the reaction is performed under conditions of 30 to 40° C. and a pH of 6 to 8 for 30 minutes to 48 hours.

9. The method of claim 7 , wherein maltooligosaccharides having a degree of polymerization of 2 to 4 are used as the substrate.

10. The method of claim 7 , wherein the monosaccharides comprise glucose or galactose.

Assignees

• Univ Korea Res & Bus Found

Inventors

• Kim Kyoung Heon
• Kim Do Hyoung

Classifications

• C12Y302/01051 Primary

  Alpha-L-fucosidase (3.2.1.51) · CPC title

• C12N15/63

  Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title

• C12Q1/34

  involving hydrolase · CPC title

• C12R2001/19

  Escherichia coli · CPC title

• C12P19/04

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title