Process of extracting oil from thin stillage

Patent metadata

Field	Value
Publication number	US-12410414-B2
Application number	US-202318167707-A
Country	US
Kind code	B2
Filing date	Feb 10, 2023
Priority date	Dec 22, 2015
Publication date	Sep 9, 2025
Grant date	Sep 9, 2025

Abstract

A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e′) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a phospholipase is present and/or added during steps (a) to (c). Use of phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.

First claim

The invention claimed is:

1. A process of recovering oil, comprising:
(a) converting a starch-containing material into dextrins with an alpha-amylase at a temperature above the initial gelatinization temperature;
(b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar;
(c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(f) optionally concentrating the thin stillage into syrup; and
(g) recovering oil from the thin stillage and/or optionally the syrup,
wherein a phospholipase C having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 2 is present and/or added during steps (b) and/or (c).

2. The process of claim 1 , wherein the phospholipase C is added during step (b).

3. The process of claim 1 , wherein the phospholipase C is added during step (c).

4. The process of claim 1 , wherein the saccharifying step (b) and fermenting step (c) are performed as a simultaneous saccharification and fermentation (SSF) and the phospholipase C is added during SSF.

5. The process of claim 1 , wherein a surfactant is present and/or added during steps (b) and/or (c).

6. The process of claim 5 , wherein the surfactant is selected from the group consisting of: polyethylene glycol, sorbitan monooleate, sorbitane monooleate, and mixtures thereof.

7. The process of claim 1 , wherein the phospholipase C has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2.

8. The process of claim 1 , wherein the phospholipase C has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.

9. A process of recovering oil, comprising:
(a) converting a starch-containing material into dextrins with an alpha-amylase at a temperature below the initial gelatinization temperature;
(b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar;
(c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(f) optionally concentrating the thin stillage into syrup; and
(g) recovering oil from the thin stillage and/or optionally the syrup,
wherein a phospholipase C having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 2 is present and/or added during steps (a), (b) and/or (c).

10. The process of claim 9 , wherein steps (b) and (c) are carried out simultaneously.

11. The process of claim 10 , wherein the phospholipase C is added during step (a).

12. The process of claim 11 , wherein a surfactant is present and/or added during step (a).

13. The process of claim 10 , wherein the phospholipase C is added during step (b) and/or step (c).

14. The process of claim 13 , wherein a surfactant is present and/or added during step (b) and/or step (c).

15. The process of claim 9 , wherein steps (a) to (c) are carried out simultaneously and the phospholipase is added during steps (a) to (c).

16. The process of claim 15 , wherein a surfactant is present and/or added during steps (a), (b) and/or (c).

17. The process of claim 9 , wherein the phospholipase C has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2.

18. The process of claim 9 , wherein the phospholipase C has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.

19. The process of claim 9 , wherein the phospholipase C comprises the amino acid sequence of SEQ ID NO: 2.

20. The process of claim 1 , wherein the phospholipase C comprises the amino acid sequence of SEQ ID NO: 2.

Assignees

• Novozymes As

Inventors

• Akerman Michael John
• Kreel Nathaniel Edward
• Hooss Melissa Carrie
• Shen Xinyu

Classifications

• C12Y301/04011

  Phosphoinositide phospholipase C (3.1.4.11) · CPC title

• C12N9/2417

  from microbiological source · CPC title

• C12N9/16 Primary

  acting on ester bonds (3.1) · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title

• C12N9/20

  Triglyceride splitting, e.g. by means of lipase · CPC title