Polypeptides having phospholipase C activity and polynucleotides encoding same
US-10745676-B2 · Aug 18, 2020 · US
Yeast expressing a heterologous phospholipase for ethanol production
US11807889B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11807889-B2 |
| Application number | US-201917283760-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 7, 2019 |
| Priority date | Oct 8, 2018 |
| Publication date | Nov 7, 2023 |
| Grant date | Nov 7, 2023 |
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Abstract
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Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding a phospholipase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
First claim
Opening claim text (preview).
The invention claimed is: 1. A recombinant Saccharomyces yeast cell comprising a heterologous polynucleotide encoding a phospholipase and a heterologous polynucleotide encoding a glucoamylase, wherein the phospholipase has a mature polypeptide sequence at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 237. 2. The recombinant yeast cell of claim 1 , wherein the fermenting organism comprises a heterologous polynucleotide encoding an alpha-amylase. 3. The recombinant yeast cell of claim 1 , wherein the fermenting organism comprises a heterologous polynucleotide encoding a protease. 4. The recombinant yeast of claim 1 , wherein the cell is a Saccharomyces cerevisiae cell. 5. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 237. 6. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence having at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 237. 7. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 237. 8. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 237. 9. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence that comprises or consists of the amino acid sequence of SEQ ID NO: 237. 10. A method of producing a fermentation product from a starch-containing or cellulosic-containing material comprising: (a) saccharifying the starch-containing or cellulosic-containing material; and (b) fermenting the saccharified material of step (a) with the recombinant yeast of claim 1 . 11. The method of claim 10 , wherein saccharification of step (a) occurs on a starch-containing material, and wherein the starch-containing material is gelatinized. 12. The method of claim 10 , wherein saccharification of step (a) occurs on a starch-containing material, and wherein the starch-containing material is ungelatinized. 13. The method of claim 10 , wherein saccharification of step (a) occurs on a starch-containing material, and wherein the method comprises liquefying the starch-containing material by contacting the material with an alpha-amylase prior to saccharification. 14. The method of claim 10 , wherein liquefying the starch-containing material and/or saccharifying the starch-containing material is conducted in presence of exogenously added protease. 15. The method of claim 10 , wherein the recombinant yeast comprises a heterologous polynucleotide encoding an alpha-amylase. 16. The method of claim 10 , wherein the recombinant yeast is a Saccharomyces cerevisiae cell. 17. The method of claim 10 , wherein the method results in higher yield of fermentation product and/or reduced foam accumulation when compared to the same process using an identical cell without the heterologous polynucleotide encoding the phospholipase under the same conditions. 18. The method of claim 10 , wherein the phospholipase of the recombinant yeast has a mature polypeptide sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 237. 19. The method of claim 10 , wherein the phospholipase of the recombinant yeast has a mature polypeptide sequence having at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 237. 20. The method of claim 10 , wherein the phospholipase of the recombinant yeast has a mature polypeptide sequence that comprises or consists of the amino acid sequence of SEQ ID NO: 237.
Assignees
Inventors
Classifications
- C12P7/06Primary
Ethanol, i.e. non-beverage · CPC title
acting on ester bonds (3.1) · CPC title
Carboxylic ester hydrolases {(3.1.1)} · CPC title
Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title
Phospholipase A2 (3.1.1.4) · CPC title
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Frequently asked questions
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- What does patent US11807889B2 cover?
- Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding a phospholipase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
- Who is the assignee on this patent?
- Novozymes As
- What technology area does this patent fall under?
- Primary CPC classification C12P7/06. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 07 2023 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).