Brassica oleracea plants with improved nutritional value

Patent metadata

Field	Value
Publication number	US-12371704-B2
Application number	US-202418405535-A
Country	US
Kind code	B2
Filing date	Jan 5, 2024
Priority date	Sep 13, 2012
Publication date	Jul 29, 2025
Grant date	Jul 29, 2025

Abstract

The invention provides compositions and methods relating to the elevation of glucoraphanin compared to standard Brassica oleracea varieties. The invention also relates to the production of hybrid varieties having desired glucosinolate contents. The invention further provides plants, plant parts, and seeds comprising such traits and comprising a Myb28 allele from Brassica villosa that is not genetically linked to an ELONG allele from Brassica villosa.

First claim

What is claimed is:

1. A method for producing a hybrid Brassica oleracea plant with elevated glucosinolate content comprising crossing a first Brassica oleracea parent plant with a second Brassica oleracea plant of a different genotype, wherein the first parent plant comprises a Myb28 allele from Brassica villosa that lacks an ELONG allele from Brassica villosa genetically linked to said Myb28 allele, wherein the Myb28 allele confers elevated glucosinolates relative to a plant lacking the Myb28 allele, and wherein a sample of seed comprising said Myb28 allele is deposited under ATCC Accession No. PTA-13165.

2. The method of claim 1 , further comprising producing a plurality of hybrid Brassica oleracea plants comprising crossing the first Brassica oleracea parent plant with a plurality of second Brassica oleracea plants of different genotypes.

3. A method of producing a Brassica oleracea plant with a desired elevated glucosinolate content comprising introgressing into the plant a chromosomal segment comprising a Myb28 allele from Brassica villosa and lacking an ELONG allele from Brassica villosa genetically linked to said Myb28 allele, wherein segment confers a desired glucosinolate content relative to a plant lacking the segment, wherein a sample of seed comprising the chromosomal segment is deposited under ATCC Accession No PTA-13165.

Assignees

• Seminis Vegetable Seeds Inc
• Plant Bioscience Ltd

Inventors

• Van Den Bosch Franciscus G
• Koorevaar Gerard N
• Mithen Richard F

Classifications

• C12Q2600/158

  Expression markers · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q2600/13

  Plant traits · CPC title

• A01H5/10

  Seeds · CPC title

• A01H1/04

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title