Brassica oleracea plants with improved nutritional value

Patent metadata

Field	Value
Publication number	US-10385353-B2
Application number	US-201615359429-A
Country	US
Kind code	B2
Filing date	Nov 22, 2016
Priority date	Sep 13, 2012
Publication date	Aug 20, 2019
Grant date	Aug 20, 2019

Abstract

The invention provides compositions and methods relating to the elevation of glucoraphanin compared to standard Brassica oleracea varieties. The invention also relates to the production of hybrid varieties having desired glucosinolate contents. The invention further provides plants, plant parts, and seeds comprising such traits and comprising a Myb28 allele from Brassica villosa that is not genetically linked to an ELONG allele from Brassica villosa.

First claim

What is claimed is:

1. A method for obtaining a Brassica plant comprising a desired glucosinolate composition, said method comprising:
(a) obtaining a Brassica plant heterozygous for a Myb28 allele from Brassica villosa that confers elevated glucosinolates relative to a Brassica oleracea plant lacking the Myb28 allele and is genetically linked in the plant to an elongation (ELONG) allele from Brassica villosa;
(b) obtaining progeny of the plant; and
(c) selecting at least a first progeny plant in which recombination has occurred such that the progeny comprises the Myb28 allele but not the ELONG allele from Brassica villosa , wherein the progeny plant possesses a desired glucosinolate composition as a result of the presence of the Myb28 allele but not the ELONG allele from Brassica villosa.

2. The method of claim 1 , wherein selecting the progeny plant comprises identifying a progeny plant that (1) comprises a genetic marker genetically linked to the Myb28 allele in Brassica villosa and/or lacks a genetic marker present at the corresponding locus in said Brassica plant, and (2) lacks a genetic marker genetically linked to the ELONG allele from Brassica villosa and/or comprises a genetic marker present at the corresponding locus from said Brassica plant.

3. The method of claim 2 , wherein selecting a progeny plant comprises detecting a polymorphism that is found in the genome of said plant flanked by the complements of SEQ ID NO:1 and SEQ ID NO:2.

4. The method of claim 3 , wherein the allele(s) are detected by a PCR-based method using oligonucleotide primer pair(s).

5. The method of claim 2 , wherein selecting a progeny plant comprises detecting in said plant a locus that is polymorphic between SEQ ID NO:8 and SEQ ID NO:9.

6. The method of claim 1 , wherein the Brassica plant is a B. oleracea plant.

Assignees

• Seminis Vegetable Seeds Inc
• Plant Bioscience Ltd

Inventors

• Van Den Bosch Franciscus G
• Koorevaar Gerard N
• Mithen Richard F

Classifications

• C12Q2600/158

  Expression markers · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12N15/8246 Primary

  Non-starch polysaccharides, e.g. cellulose, fructans, levans · CPC title