Activation of bioluminescence by structural complementation

Patent metadata

Field	Value
Publication number	US-12366572-B2
Application number	US-202418890453-A
Country	US
Kind code	B2
Filing date	Sep 19, 2024
Priority date	Mar 15, 2013
Publication date	Jul 22, 2025
Grant date	Jul 22, 2025

Abstract

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

First claim

The invention claimed is:

1. A composition comprising:
(a) a polypeptide comprising SEQ ID NO: 1270;
(b) a first peptide comprising an amino acid sequence having 100% sequence identity with SEQ ID NO: 2587, wherein the first peptide is conjugated to a first binding moiety; and
(c) a second peptide comprising an amino acid sequence having 100% sequence identity with SEQ ID NO: 374, wherein the second peptide is conjugated to a second binding moiety.

2. The composition of claim 1 , wherein the first and second binding moieties are antibodies or antibody fragments.

3. The composition of claim 2 , wherein the first and second binding moieties are monoclonal antibodies (mAb) or single-chain variable fragments (scFvs).

4. The composition of claim 3 , wherein the first and second binding moieties recognize different epitopes on a target molecule.

5. A method of detecting a target molecule in a sample comprising:
(a) contacting the sample with the composition of claim 4 and a furimazine substrate; and
(b) detecting luminescence produced by a complex formed from the first peptide, the second peptide, and the polypeptide; wherein the amount of luminescence is directly proportional to the target concentration in the test sample.

Assignees

• Promega Corp

Inventors

• Dixon Andrew S
• Encell Lance
• Hall Mary
• Wood Keith
• Wood Monika
• Schwinn Marie
• Binkowski Brock F
• Zegzouti Hicham
• Nath Nidhi
• Mondal Subhanjan
• Goueli Said
• Meisenheimer Poncho
• Kirkland Thomas
• Unch James
• Pulukkunat Dileep K
• Robers Matthew
• Dart Melanie
• Machleidt Thomas

Classifications

• A61K51/08

  Peptides, e.g. proteins {, carriers being peptides, polyamino acids, proteins} · CPC title

• H04W4/02

  Services making use of location information · CPC title

• G06Q30/0631

  Recommending goods or services · CPC title

• G06Q30/0283

  Price estimation or determination · CPC title

• H04W4/35

  for the management of goods or merchandise · CPC title