Standardizing diffusion of a fluid into tissue

Patent metadata

Field	Value
Publication number	US-12360079-B2
Application number	US-202117505095-A
Country	US
Kind code	B2
Filing date	Oct 19, 2021
Priority date	Feb 9, 2015
Publication date	Jul 15, 2025
Grant date	Jul 15, 2025

Abstract

Disclosed are a system and method for evaluating a tissue sample that has been removed from a subject. Movement of fluid through the tissue sample is monitored by measuring time of flight of acoustic waves passed through the tissue sample. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. Using the disclosed method and system, an optimized protocol for ensuring adequate distribution of the fluid throughout a variety of tissue types and/or sample sizes can be developed and utilized.

First claim

The invention claimed is:

1. A system for optimizing a fixation of a tissue sample having a first tissue type, the system comprising:
(a) an acoustic device, wherein the acoustic device comprises one or more transmitters, wherein the one or more transmitters are configured to emit ultrasonic waves at an intensity of less than 1 W/cm 2 , and one or more receivers configured to detect the ultrasonic waves emitted by the one or more transmitters; and
(b) a computing device communicatively coupled to the acoustic monitoring device, where the computing device comprises (i) one or more processors, and (ii) one or more memories coupled to the one or more processors, the one or more memories storing computer-executable instructions that, when executed by the one or more processors, cause the system to perform operations comprising:
(a) monitoring a diffusion of a fluid at one or more temperatures through the tissue sample having the first tissue type using the acoustic device, wherein the monitoring of the diffusion of the fluid comprises repeatedly measuring in real-time a time-of-flight of the emitted ultrasonic waves that have traveled through the tissue sample having the first tissue type while the tissue sample having the first tissue type is immersed within the fluid; and
(b) determining a time when the fluid has diffused through at least a predetermined portion of the tissue sample having the first tissue type based on the monitoring of the diffusion of the fluid at the one or more temperatures through the tissue sample having the first tissue type.

2. The system of claim 1 , wherein the operations further comprise computing a decay curve constant based on the repeated monitoring of the diffusion of the fluid through the tissue sample having the first tissue type.

3. The system of claim 1 , wherein the operations further comprise storing the computed decay constant in a record of a tissue type of the tissue sample having the first tissue type.

4. The system of claim 3 , wherein the operations further comprise processing based on the decay constant subsequent tissue samples of a same type as the tissue sample having the first tissue type.

5. The system of claim 1 , wherein the fluid is a tissue fixative.

6. The system of claim 5 , wherein the tissue fixative comprises an aldehyde-based tissue fixative.

7. The system of claim 6 , wherein the aldehyde-based tissue fixative comprises formaldehyde.

8. The system of claim 1 , wherein the fluid is at a temperature ranging from between about 0° C. to 15° C.

9. The system of claim 1 , wherein the fluid is at a temperature ranging from between about 0° C. to 10° C.

10. The system of claim 1 , wherein the fluid is at a temperature ranging from between about 5° C. to 15° C.

11. The system of claim 1 , wherein the ultrasonic waves have an intensity of less than 0.5 W/cm 2 .

12. The system of claim 1 , wherein the tissue sample having the first tissue type is less than 7 mm thick.

Assignees

• Ventana Med Syst Inc

Inventors

• Bauer Daniel
• Chafin David
• Otter Michael
• Theiss Abbey

Classifications

• G01N2291/024

  Mixtures · CPC title

• G01N2291/02475

  Tissue characterisation · CPC title

• G01N1/30

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title

• G01N29/07

  by measuring propagation velocity or propagation time of acoustic waves · CPC title

• G01N29/024 Primary

  by measuring propagation velocity or propagation time of acoustic waves · CPC title