Method for tissue sample fixation

Patent metadata

Field	Value
Publication number	US-10126216-B2
Application number	US-201213372040-A
Country	US
Kind code	B2
Filing date	Feb 13, 2012
Priority date	Feb 17, 2011
Publication date	Nov 13, 2018
Grant date	Nov 13, 2018

Abstract

An aldehyde fixative solution at a first temperature is caused to contact a tissue sample for a first time period, additionally an aldehyde fixative solution is caused to contact the tissue sample at a second temperature higher than the first temperature for a second time period. The first time period typically ranges from about 15 minutes up to about 4 hours, and the first temperature typically is from greater than 0° C. to at least 15° C. The second temperature typically is from greater than about 22° C. to about 55° C., and the second time period ranges from about 1 hour to about 4 hours. Using this process, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals have been accomplished in approximately 4 hours compared to 24 hours for room temperature protocols, and more even morphology and antigen preservation are observed.

First claim

We claim:

1. A method for fixing a tissue sample, consisting of:
contacting the tissue sample with a first aldehyde solution for a first time period sufficient to diffuse the first aldehyde solution into an interior region of the tissue sample, a temperature of the first aldehyde solution during the first time period being from 0° C. to 10° C.; and
removing the tissue sample from the first aldehyde solution that is in contact with the tissue sample during the first time period; and
submerging the tissue sample in a second aldehyde solution preheated to a temperature from 22° C. to 50° C. for a second time period, the second time period being sufficient to fix the tissue sample.

2. The method according to claim 1 , where the temperature of the first aldehyde solution during the first time period is from 3° C. to 5° C.

3. The method according to claim 1 , where the first time period is from 15 minutes to 4 hours.

4. The method according to claim 1 , where the first time period is from 1 hour to 2 hours.

5. The method according to claim 1 , where the temperature of the second aldehyde solution during the second time period is from 35° C. to 45° C.

6. The method according to claim 1 , where the second time period is from 1 hour to 4 hours.

7. The method according to claim 1 , where the second time period is from 2 hours to 3 hours.

8. A method for fixing a tissue sample, consisting of:
immersing the tissue sample in a first formalin solution at a temperature from 0° C. to 5° C. for a first time period from 15 minutes to 4 hours; and
after the first period of time, transferring the tissue sample from the first formalin solution to a second formalin solution at a temperature from 22° C. to 55° C. and immersing the tissue sample in the second formalin solution for a second time period from about 1 hour to about 4 hours.

9. The method according to claim 1 , where the tissue sample is from 1 mm to 10 mm thick.

10. The method according to claim 8 , where the tissue sample is from 1 mm to 10 mm thick.

11. The method according to claim 1 , where the first time period is sufficient to diffuse the formalin solution throughout substantially all of the tissue sample.

12. The method according to claim 8 , where the immersing the tissue sample in the second formalin solution includes immersing the tissue sample in the second formalin solution at a temperature from 35° C. to 45° C. for the second time period.

13. The method of claim 1 , where at least 50% of post-translational modification signals within the tissue sample before contacting the tissue sample and the first aldehyde solution for the first time period are preserved after fixing the tissue sample for the second time period.

14. A method for processing a tissue sample, consisting of:
contacting the tissue sample and a first formalin solution at a temperature from 0° C. to 10° C. for a first time period sufficient to diffuse the first formalin solution throughout substantially all of the tissue sample to obtain a formalin diffused tissue sample;
transferring the formalin-diffused tissue sample to a second formalin solution, the second formalin solution being at a temperature from 35° C. to 45° C., and holding the formalin -diffused tissue sample in the second formalin solution for a second time period from 1 hour to 4 hours to obtain a fixed tissue sample; and
immunohistochemically staining the fixed tissue sample,
where the tissue sample is from 1 mm to 10 mm thick and at least 50% of post-translational modification signals within the tissue sample before contacting the tissue sample and the first formalin solution for the first time period are preserved after contacting the tissue sample and the second formalin solution for the second time period.

15. The method of claim 1 , wherein the second time period is at least one hour.

Assignees

• Chafin David
• Theiss Abbey Pierson
• Otter Michael
• Roberts Esteban
• Ventana Med Syst Inc

Inventors

• Chafin David
• Theiss Abbey Pierson
• Otter Michael
• Roberts Esteban

Classifications

• G01N1/31

  Apparatus therefor · CPC title

• G01N1/30 Primary

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title