Methods for the improved formation of acarbose

Patent metadata

Field	Value
Publication number	US-12351847-B2
Application number	US-202017769001-A
Country	US
Kind code	B2
Filing date	Oct 2, 2020
Priority date	Oct 16, 2019
Publication date	Jul 8, 2025
Grant date	Jul 8, 2025

Abstract

The present invention relates to Actinomycetales strains for the improved formation of acarbose. Provided are Actinomycetales strains which are engineered to overexpress dTDP-D-glucose-4,6-dehydratase (AcbB) and/or uridyltransferase (GtaB). Also provided are Actinomycetales strains which are engineered to have a reduced or absent expression of the small carbohydrate binding protein (Cgt) and/or a reduced or absent expression of genes which are essential for carotenoid synthesis. Also provided are tools, methods and means to generate these strains.

First claim

The invention claimed is:

1. A method to engineer an Actinoplanes strain for the improved production of acarbose, the method comprising engineering the Actinoplanes strain by deleting the gene encoding extracellular small carbohydrate binding protein Cgt according to SEQ ID No. 20.

2. An Actinoplanes strain for the improved production of acarbose, wherein the Actinoplanes strain is genetically engineered by deleting the gene encoding the extracellular small carbohydrate binding protein Cgt according to SEQ ID No. 20.

Assignees

• Bayer Ag

Inventors

• Puhler Alfred
• Kalinowski Jorn
• Schneiker-Bekel Susanne
• Persicke Marcus
• Schaffert Lena

Classifications

• C12Y402/01046

  dTDP-glucose 4,6-dehydratase (4.2.1.46) · CPC title

• C12Y207/07009

  UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase · CPC title

• C12N15/76

  for Actinomyces; for Streptomyces · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title

• C12N9/88 Primary

  Lyases (4.) · CPC title