Methods for promoting plant health using free enzymes and microorganisms that overexpress enzymes

Patent metadata

Field	Value
Publication number	US-12351533-B2
Application number	US-202217932994-A
Country	US
Kind code	B2
Filing date	Sep 16, 2022
Priority date	Mar 16, 2016
Publication date	Jul 8, 2025
Grant date	Jul 8, 2025

Abstract

Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

First claim

What is claimed is:

1. A composition comprising a fertilizer and a free enzyme, wherein the enzyme is a mannanase, and wherein the enzyme is active.

2. The composition of claim 1 , wherein the composition further comprises a biostimulant, an agriculturally acceptable carrier, an additional agrochemical, or a combination thereof.

3. The composition of claim 2 , wherein the agriculturally acceptable carrier comprises a dispersant, a surfactant, an additive, water, a thickener, an anti-caking agent, residue breakdown product, a composting formulation, a granular application, diatomaceous earth, an oil, a coloring agent, a stabilizer, a preservative, a polymer, a coating, or a combination of any thereof.

4. The composition of claim 2 , wherein the agriculturally acceptable carrier comprises vermiculite, charcoal, sugar factory carbonation press mud, rice husk, carboxymethyl cellulose, peat, perlite, fine sand, calcium carbonate, flour, alum, a starch, talc, polyvinyl pyrrolidone, or a combination of any thereof.

5. The composition of claim 2 , wherein the additional agrochemical comprises an insecticide, a nematicide, an herbicide, a plant growth amendment, a fungicide, an insecticide, a molluscicide, an algicide, a bacterial inoculant, a fungal inoculant, a plant hormone, or a combination of any thereof.

6. The composition of claim 3 , wherein the agriculturally acceptable carrier comprises an additive, and the additive comprises an oil, a gum, a resin, a clay, a polyoxyethylene glycol, a terpene, a viscid organic, a fatty acid ester, a sulfated alcohol, an alkyl sulfonate, a petroleum sulfonate, an alcohol sulfate, a sodium alkyl butane diamate, a polyester of sodium thiobutane dioate, a benzene acetonitrile derivative, a proteinaceous material, or a combination of any thereof; the agriculturally acceptable carrier comprises a thickener, and the thickener comprises a long chain alkylsulfonate of polyethylene glycol, a polyoxyethylene oleate, or a combination of any thereof; the agriculturally acceptable carrier comprises a surfactant, and the surfactant comprises a heavy petroleum oil, a heavy petroleum distillate, a polyol fatty acid ester, a polyethoxylated fatty acid ester, an aryl alkyl polyoxyethylene glycol, an alkyl amine acetate, an alkyl aryl sulfonate, a polyhydric alcohol, an alkyl phosphate, or a combination of any thereof; or the agriculturally acceptable carrier comprises an anti-caking agent, and the anti-caking agent comprises a sodium salt, a calcium carbonate, diatomaceous earth, or a combination of any thereof.

7. The composition of claim 1 , wherein the fertilizer comprises nitrogen, phosphate, potassium, zinc, iron, selenium, boron, copper, or a combination of any thereof.

8. The composition of claim 7 , wherein the phosphate comprises monoammonium phosphate, diammonium phosphate, orthophosphate, orthopolyphosphate, or a combination of any thereof; or wherein the potassium comprises potassium acetate.

9. The composition of claim 1 , wherein the enzyme comprises:
(a) a crude cell extract containing the enzyme;
(b) a partially purified enzyme; or
(c) a substantially purified enzyme.

10. The composition of claim 1 , wherein the enzyme comprises enzyme that is immobilized on a matrix or support.

11. The composition of claim 10 , wherein the matrix or support comprises charcoal, biochar, nanocarbon, agarose, an alginate, cellulose, a cellulose derivative, silica, plastic, stainless steel, glass, polystyrene, a ceramic, dolomite, a clay, diatomaceous earth, talc, a polymer, a gum, a water-dispersable material, or a combination of any thereof.

12. The composition of claim 1 , wherein the mannanase comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 128.

13. The composition of claim 1 , wherein the mannanase comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 128.

14. The composition of claim 1 , wherein the fertilizer comprises a micronutrient fertilizer material, the micronutrient fertilizer material comprising boric acid, a borate, a boron frit, copper sulfate, a copper frit, a copper chelate, a sodium tetraborate decahydrate, an iron sulfate, an iron oxide, iron ammonium sulfate, an iron frit, an iron chelate, a manganese sulfate, a manganese oxide, a manganese chelate, a manganese chloride, a manganese frit, a sodium molybdate, molybdic acid, a zinc sulfate, a zinc oxide, a zinc carbonate, a zinc frit, zinc phosphate, a zinc chelate, or a combination of any thereof.

15. The composition of claim 1 , wherein the fertilizer comprises ammonium sulfate, ammonium nitrate, ammonium sulfate nitrate, ammonium chloride, ammonium bisulfate, ammonium polysulfide, ammonium thiosulfate, aqueous ammonia, anhydrous ammonia, ammonium polyphosphate, aluminum sulfate, calcium nitrate, calcium ammonium nitrate, calcium sulfate, calcined magnesite, calcitic limestone, calcium oxide, calcium nitrate, dolomitic limestone, hydrated lime, calcium carbonate, diammonium phosphate, monoammonium phosphate, magnesium nitrate, magnesium sulfate, potassium nitrate, potassium chloride, potassium magnesium sulfate, potassium sulfate, sodium nitrates, magnesian limestone, magnesia, urea, urea-formaldehydes, urea ammonium nitrate, sulfur-coated urea, polymer-coated urea, isobutylidene diurea, K 2 SO 4 -2MgSO 4 , kainite, sylvinite, kieserite, Epsom salts, elemental sulfur, marl, ground oyster shells, fish meal, oil cakes, fish manure, blood meal, rock phosphate, super phosphates, slag, bone meal, wood ash, manure, bat guano, peat moss, compost, green sand, cottonseed meal, feather meal, crab meal, fish emulsion, humic acid, or a combination of any thereof.

16. The composition of claim 15 , wherein the fertilizer comprises 12% ammoniacal nitrogen and 58% available phosphate.

17. A method for stimulating plant growth and/or promoting plant health, comprising applying the composition of claim 1 to a plant growth medium, a plant, a plant seed, or an area surrounding a plant or a plant seed.

18. A plant seed treated with the composition of claim 1 .

19. The plant seed of claim 18 , wherein the plant seed is coated with the composition.

20. The composition of claim 1 , wherein the composition is a granular composition.

21. The composition of claim 1 , wherein the composition further comprises a phospholipase, a lipase, a xylanase, a xylosidase, a lactonase, a pectinase, a chitosanase, a protease, an acid phosphatase, a non-cellulolytic glucanase, an ACC deaminase, a phytase, or combinations of any thereof.

Assignees

• Spogen Biotech Inc

Inventors

• Thompson Brian M
• Augustin Jorg

Classifications

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8249

  involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life · CPC title

• C12N15/8237

  Externally regulated expression systems · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C07K14/415

  from plants · CPC title