High expression cereal phytase gene

Patent metadata

Field	Value
Publication number	US-9540633-B2
Application number	US-201214110763-A
Country	US
Kind code	B2
Filing date	Apr 25, 2012
Priority date	Apr 27, 2011
Publication date	Jan 10, 2017
Grant date	Jan 10, 2017

Abstract

The present invention provides mutant cereal plants and mature grain thereof, characterised by enhanced levels of the enzyme phytase in the grain, and methods for inducing, detecting and selecting the mutant cereal plants. The invention further relates to animal feed comprising said grain having enhanced amounts of phytase.

First claim

The invention claimed is:

1. A method for producing a Triticum plant capable of producing an average phytase endosperm content of greater than 4300 FTU/kg, said method comprising:
a) obtaining a sample of nucleic acids from a Triticum plant or portion thereof;
b) detecting in said sample the presence of the nucleotide V at the 5′ end of a polynucleotide having the nucleotide sequence ACA VGA GTC ATG CAT (SEQ ID NO:1), wherein V is a cytosine;
c) breeding a Triticum plant comprising said nucleotide sequence with a second Triticum plant to obtain grains; and
d) growing at least one Triticum plant from said grains;
wherein said Triticum plant grown from said grains comprises said nucleotide V at the 5′ end of a polynucleotide having the nucleotide sequence ACA VGA GTC ATG CAT (SEQ ID NO: 1), and wherein said V is a cytosine.

2. The method according to claim 1 , wherein said sample of nucleic acids comprises a first polynucleotide located 5′ upstream of and operably linked to a second polynucleotide, wherein said first polynucleotide comprises the nucleotide sequence ACA VGA GTC ATG CAT (SEQ ID NO:1), wherein V is a cytosine, and wherein said second polynucleotide encodes a phytase polypeptide having myo-inositol hexakisphosphate phosphohydrolase activity.

3. The method according to claim 1 , wherein a population of Triticum plants grown from said grains have an average phytase grain content of greater than 4300 FTU/kg.

4. The method of claim 1 wherein said polynucleotide is operably linked to a phytase encoding sequence.

Assignees

• Brinch-Pedersen Henrik
• Madsen Claus K
• Dionisio Giuseppe
• Holm Preben Bach
• Univ Aarhus

Inventors

• Brinch-Pedersen Henrik
• Madsen Claus K
• Dionisio Giuseppe
• Holm Preben Bach

Classifications

• C12N15/8234

  Seed-specific, e.g. embryo, endosperm · CPC title

• C12N9/16 Primary

  acting on ester bonds (3.1) · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12N15/01 Primary

  Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor · CPC title

• C12N15/8243

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title