Methods for promoting plant health using free enzymes and microorganisms that overexpress enzymes

Patent metadata

Field	Value
Publication number	US-12351532-B2
Application number	US-202117459031-A
Country	US
Kind code	B2
Filing date	Aug 27, 2021
Priority date	Mar 16, 2016
Publication date	Jul 8, 2025
Grant date	Jul 8, 2025

Abstract

Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

First claim

What is claimed is:

1. A method for stimulating plant growth and/or promoting plant health comprising applying a free enzyme to a plant growth medium, a plant seed, or an area surrounding a plant or a plant seed, wherein the enzyme comprises a Triticum aestivum acid phosphatase; and
wherein the plant or a plant produced from the plant seed exhibits an increase in plant height.

2. The method of claim 1 , wherein the method further comprises applying at least a second free enzyme selected from a phospholipase, a lipase, a xylanase, a xylosidase, a lactonase, a chitosanase, a protease, a phytase, a mannanase, a pectinase, a glucanase, and an ACC deaminase.

3. The method of claim 1 , wherein the method comprises applying the enzyme to the plant growth medium.

4. The method of claim 3 , wherein the plant growth medium comprises a fertilizer.

5. The method of claim 3 , wherein the plant growth medium consists essentially of a fertilizer.

6. The method of claim 1 , wherein the method comprises applying the enzyme to the plant.

7. The method of claim 6 , wherein the method comprises applying the enzyme to roots of the plant.

8. The method of claim 1 , wherein the enzyme comprises:
a crude cell extract containing the enzyme;
a partially purified enzyme;
a substantially purified enzyme; or
an enzyme that is immobilized on a matrix or support.

9. The method of claim 1 , wherein applying the enzyme comprises an in-furrow treatment.

10. The method of claim 1 , wherein the acid phosphatase comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 130 or 131.

11. The method of claim 1 , wherein the method further comprises applying a agrochemical.

12. The method of claim 1 , wherein the applying the enzyme comprises applying a liquid solution comprising the enzyme.

13. The method of claim 1 , wherein the applying the enzyme comprises applying a powder agent comprising the enzyme.

14. The method of claim 1 , wherein the applying the enzyme comprises applying a granular formulation comprising the enzyme.

15. The method of claim 1 , wherein the acid phosphatase comprises an amino acid sequence having SEQ ID NO: 130 or 131.

16. The method of claim 2 , wherein:
the phospholipase comprises an amino acid sequence having at least 80% identity to any one of SEQ ID NOs. 13-19 and 115-117;
the lipase comprises an amino acid sequence having at least 80% identity to any one of SEQ ID NOs: 20-21 and 118-120;
the mannanase comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 128;
the pectinase comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 129; or
the xylanase comprises an amino acid sequence having at least 80% identity to any one of SEQ ID NOs: 22-25 and 121-122.

17. The method of claim 2 , wherein the second free enzyme comprises the mannanase, and wherein the mannanase comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 128.

18. The method of claim 1 , wherein the acid phosphatase comprises a Triticum aestivum acid phosphatase.

19. The method of claim 2 , wherein the acid phosphatase and the at least second enzyme are present in synergistically effective amounts.

20. The method of claim 3 , wherein the method comprises applying the enzyme and applying a fertilizer to the plant growth medium.

21. The method of claim 20 , wherein the fertilizer comprises urea, superphosphate, a chelated micronutrient, a chelating agent, monoammonium phosphate, or diammonium phosphate.

22. The method of claim 20 , wherein the fertilizer comprises a nitrogen stabilizer, a polymer, a microbe, or a microbial extract.

23. The method of 2 , wherein the method comprises applying the enzymes to the plant growth medium.

24. The method of claim 23 , wherein the plant growth medium comprises a fertilizer.

25. The method of claim 23 , wherein the plant growth medium consists essentially of a fertilizer.

26. The method of claim 23 , wherein the method comprises applying the enzymes and applying a fertilizer to the plant growth medium.

27. The method of claim 26 , wherein the fertilizer comprises urea, superphosphate, a chelated micronutrient, a chelating agent, monoammonium phosphate, or diammonium phosphate.

28. The method of claim 26 , wherein the fertilizer comprises a nitrogen stabilizer, a polymer, a microbe, or a microbial extract.

29. The method of claim 2 , wherein the method comprises applying the enzyme to roots of the plant.

30. The method of claim 2 , wherein the acid phosphatase comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 130 or 131.

31. The method of claim 2 , wherein applying the enzymes comprises applying a granular formulation comprising the enzymes.

Assignees

• Spogen Biotech Inc

Inventors

• Thompson Brian M
• Augustin Jorg

Classifications

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8249

  involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life · CPC title

• C12N15/8237

  Externally regulated expression systems · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C07K14/415

  from plants · CPC title