Method of dough relaxation involving endopeptidases

Patent metadata

Field	Value
Publication number	US-12349684-B2
Application number	US-202017625205-A
Country	US
Kind code	B2
Filing date	Aug 5, 2020
Priority date	Aug 7, 2019
Publication date	Jul 8, 2025
Grant date	Jul 8, 2025

Abstract

A method for increasing the softness and the relaxation of a dough comprising a) adding at least one endopeptidase having at least 60% identity to SEQ ID NO:1 or SEQ ID NO:2 to flour or to a dough comprising a flour, wherein no L-cystein and/or sodium metabisulfite is added to the dough; b) kneading the dough; and c) making the dough into an edible product.

First claim

The invention claimed is:

1. A method for increasing softness and relaxation of a dough, comprising
a) adding at least one endopeptidase having at least 60% identity to SEQ ID NO:1 or SEQ ID NO:2 to flour or to a dough comprising a flour, wherein no L-cystein and/or sodium metabisulfite is added to the dough;
b) kneading the dough; and
c) making the dough into an edible product;
wherein the dough has increased softness and relaxation compared to a dough which is prepared under the same conditions but no endopeptidase having at least 60% identity to SEQ ID NO: 1 or SEQ ID NO: 2 is added to the flour or dough comprising a flour.

2. The method according to claim 1 , wherein the edible product is selected from the group consisting of bread, flat bread, buns, crackers, pasta, noodles, laminated baking products, biscuits, baguettes, and pizzas.

3. The method according to claim 1 , wherein the edible product is biscuits.

4. The method according to claim 1 , wherein the flour is selected from the group consisting of wheat flour, corn flour, rye flour, barley flour, oat flour, rice flour, sorghum flour, and a combination thereof.

5. The method according to claim 1 , wherein the dough has increased elasticity compared to a dough prepared under the same conditions but wherein no endopeptidase having at least 60% identity to SEQ ID NO: 1 or SEQ ID NO:2 is added to the flour or dough comprising a flour.

6. The method according to claim 1 , wherein the at least one endopeptidase is added in an amount of 0.1-1000 mg of enzyme protein per kg of flour.

7. The method according to claim 1 , wherein the dough has improved extensibility compared to a dough prepared under the same conditions but wherein no endopeptidase having at least 60% identity to SEQ ID NO: 1 or SEQ ID NO: 2 is added to the flour or dough comprising a flour.

8. The method according to claim 1 , wherein the dough further comprises one or more enzymes selected from the group consisting of amylase, maltogenic amylase, beta amylase, aminopeptidase, carboxypeptidase, catalase, cellulytic enzyme, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, glucan 1,4-alpha-maltotetrahydrolase, glucanase, galactanase, alpha-galactosidase, beta-galactosidase, glucoamylase, glucose oxidase, alpha-glucosidase, beta-glucosidase, haloperoxidase, hemicellulytic enzyme, invertase, laccase, lipase, mannanase, mannosidase, oxidase, pectinolytic enzymes, peptidoglutaminase, peroxidase, phospholipase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, and xylanase.

9. The method according to claim 1 , wherein the at least one endopeptidase has at least 70% identity to SEQ ID NO: 1 or SEQ ID NO: 2.

10. The method according to claim 1 , wherein the at least one endopeptidase has at least 80% identity to SEQ ID NO: 1 or SEQ ID NO: 2.

11. The method according to claim 1 , wherein the at least one endopeptidase has at least 90% identity to SEQ ID NO: 1 or SEQ ID NO: 2.

12. The method according to claim 1 , wherein the at least one endopeptidase has at least 95% identity to SEQ ID NO: 1 or SEQ ID NO: 2.

13. The method according to claim 1 , wherein the at least one endopeptidase is the endopeptidase of SEQ ID NO: 1 or SEQ ID NO: 2.

Assignees

• Novozymes As

Inventors

• Matveeva Irina Victorovna
• Puder Katja
• Akbar Sajid

Classifications

• C12Y304/21004 Primary

  Trypsin (3.4.21.4) · CPC title

• C12N9/50

  Proteinases {, e.g. Endopeptidases (3.4.21-3.4.25)} · CPC title

• C12Y304/21

  Serine endopeptidases (3.4.21) · CPC title

• A21D8/042 Primary

  with enzymes · CPC title

• A21D2/26 Primary

  Proteins · CPC title