Method for extracting gelatin

Patent metadata

Field	Value
Publication number	US-12344769-B2
Application number	US-202017439859-A
Country	US
Kind code	B2
Filing date	Apr 7, 2020
Priority date	Apr 8, 2019
Publication date	Jul 1, 2025
Grant date	Jul 1, 2025

Abstract

Provided is an enzymatic method of extracting high quality gelatin from collagen containing material.

First claim

What is claimed is:

1. A method for extracting gelatin, comprising:
(a) demineralizing a collagen containing material to produce ossein;
(b) grinding the ossein to particles that can pass 10 meshes sieves;
(c) incubating the ground ossein in the presence of an effective amount of a protease, and
(d) extracting the gelatin from the ground ossein, wherein the protease is a metalloprotease belonging to family M35 and comprises of an amino acid sequence having at least 85% identity to amino acids 1-177 of the amino acid sequence SEQ ID NO: 1.

2. The method of claim 1 , further comprising, after the incubation and before the extraction, raising the temperature for at least 10 minutes to deactivate the protease.

3. The method of claim 1 , further comprising, after the incubation and before the extraction, raising the temperature for at least 5 minutes to deactivate the protease.

4. The method of claim 1 , wherein the extraction is a multi-grade extraction, comprising extraction of the gelatin during a step-by-step heating process, wherein the extraction is performed at a temperature of between 50° C. and 100° C.

5. The method of claim 1 , further comprising, prior to (a), adding an acid to the collagen containing material for demineralizing.

6. The method of claim 1 , wherein the incubation is performed at a temperature of between 20° C. to 40° C., and/or at a pH of between 4 and 6.

7. The method of claim 1 , wherein the protease comprises an amino acid sequence having at least 90% identity to amino acids 1-177 of the amino acid sequence SEQ ID NO: 1.

8. The method of claim 1 , wherein the protease comprises an amino acid sequence having at least 95% identity to amino acids 1-177 of the amino acid sequence SEQ ID NO: 1.

9. The method of claim 1 , wherein the protease comprises an amino acid sequence having at least 99% identity to amino acids 1-177 of the amino acid sequence SEQ ID NO: 1.

Assignees

• Novozymes As

Inventors

• Wang Mingzhu
• Zhang Yu
• Wang Yazhen

Classifications

• C09H7/00

  Preparation of water-insoluble gelatine · CPC title

• C12P21/06

  produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00) · CPC title

• C09H3/00 Primary

  Isolation of glue or gelatine from raw materials, e.g. by extracting, by heating (gelatine for foodstuffs A23J1/10) · CPC title

• C08L89/06 Primary

  derived from leather or skin {, e.g. gelatin} · CPC title