Variant maltohexaose-forming α-amylase variants

US12338467B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-12338467-B2
Application numberUS-202418405674-A
CountryUS
Kind codeB2
Filing dateJan 5, 2024
Priority dateOct 28, 2011
Publication dateJun 24, 2025
Grant dateJun 24, 2025

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Abstract

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Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.

First claim

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What is claimed is: 1. A variant α-amylase polypeptide derived from a parental α-amylase polypeptide, comprising at least two combinable mutations at productive amino acid positions; wherein the mutations are at position Q169 and E391, wherein the variant has at least 90% amino acid sequence identity with SEQ ID NO: 3. 2. The variant amylase of claim 1 , wherein the mutations are Q169E and E391A. 3. The variant amylase of claim 1 , further comprising a plurality of combinable mutations. 4. The variant amylase of claim 1 , further comprising a deletion corresponding to one or more residue selected from the group consisting of Arg-181, Gly-182, His-183, and Gly-184, using SEQ ID NO: 3 for numbering. 5. The variant amylase of claim 1 , further comprising deletions corresponding to residues Arg-181 and Gly-182, using SEQ ID NO: 3 for numbering. 6. A composition comprising the variant amylase of claim 1 . 7. The composition of claim 6 , wherein the composition is effective for removing starchy stains from laundry, dishes, or textiles. 8. The composition of claim 6 , further comprising a surfactant.

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What does patent US12338467B2 cover?
Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Who is the assignee on this patent?
Danisco Us Inc
What technology area does this patent fall under?
Primary CPC classification C12N9/2417. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Jun 24 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).