Who is the assignee on this patent?

Jackson Michelle, Souter Philip Frank, Bewick Lindsay Suzanne, and 14 more

What technology area does this patent fall under?

Primary CPC classification C12N9/2417. Mapped technology areas include Chemistry & Metallurgy.

When was this patent published?

Publication date Tue Mar 15 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.

What related patents are in patentsdb?

We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).

Cleaning compositions comprising amylase variants reference to a sequence listing

US9284544B2 · US · B2

Patent metadata
Field	Value
Publication number	US-9284544-B2
Application number	US-201213537606-A
Country	US
Kind code	B2
Filing date	Jun 29, 2012
Priority date	Jun 30, 2011
Publication date	Mar 15, 2016
Grant date	Mar 15, 2016

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Title
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Abstract
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First independent claim
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Abstract

Official abstract text for this publication.

The present invention relates to cleaning compositions comprising variants of an alpha-amylase and methods of treating surfaces such as textiles with aqueous liquor comprising such compositions, especially at low temperatures.

First claim

Opening claim text (preview).

What is claimed is: 1. A cleaning composition comprising: (a) a variant of a parent alpha-amylase comprising an amino acid substitution selected from the group consisting of E260G, E260H, E260I, E260K, E260R, E260T, and E260Y at a position corresponding to position E260 of the mature polypeptide of SEQ ID NO:1 and further comprising an amino acid sequence alteration at one or more positions corresponding to positions D134, W140, W189, F262, W284, G304, W347, W439, W469, G476, and G477 of the mature polypeptide of SEQ ID NO:1, wherein each alteration is independently an amino acid substitution, deletion or insertion, and wherein the variant has at least 80%, but less than 100% sequence identity with the mature polypeptide of SEQ ID NO:1, and wherein the variant has alpha-amylase activity; and (b) a cleaning adjunct in an amount from 0.01 to 99.9 wt %. 2. A composition according to claim 1 , wherein the further amino acid sequence alteration is a substitution. 3. A composition according to claim 1 , wherein the variant comprises substitutions at three positions selected from the group consisting of G304, W140, W189, D134, E260, F262, W284, W347, W439, W469, G476, and G477. 4. A composition according to claim 1 , wherein the variant comprises substitutions at four positions selected from the group consisting of G304, W140, W189, D134, E260, F262, W284, W347, W439, W469, G476, and G477. 5. A composition according to claim 1 , wherein the variant comprises substitutions at two, three or four positions selected from the group consisting of G304, W140, E260 and G476. 6. A composition according to claim 1 , wherein the variant comprises a substitution selected from the group consisting of E260G, E260H, E260I, E260K, E260R, E260T, and E260Y and further comprises one or more substitutions selected from the group consisting of G304R, W140Y, W140F, W189E, W189G, W189T, D134E, W284D, W284F, W284R, W439R, W439G, G476E, G476K, G477E, G477K, G477M, and G477R. 7. A composition according to claim 1 , wherein the variant comprises a substitution selected from the group consisting of E260G, E260H, E260I, E260K, E260R, E260T, and E260Y and further comprises substitutions at one, two, or three positions selected from the group consisting of G304R, W140Y, W140F, G476E, and G476K. 8. A composition according to claim 7 , wherein the substitutions are selected from the group consisting of G304R, W140Y, E260G and G476K. 9. A composition according to claim 1 wherein the variant further comprises one or more substitutions selected from the group consisting of N195F, V206Y, Y243F, G109A, G273D, G273V, G337N, K72R, R181H, S303G and Y100I. 10. A composition according to claim 1 wherein the number of alterations in the variant is from 2 to 10. 11. A composition according to claim 1 , wherein the variant alpha-amylase has at least 87%, but less than 100%, sequence identity with the mature polypeptide of SEQ ID NO:1. 12. A composition according to claim 1 , wherein the variant further comprises substitutions at positions corresponding to the positions of the polypeptide of SEQ ID NO:1, selected from the group consisting of: W140Y+N195F+V206Y+Y243F+E260G+G477E, W140Y+N195F+V206Y+Y243F+E260T+W284D, G109A+W140Y+N195F+V206Y+Y243F+E260G, W140Y+N195F+V206Y+Y243F+E260G, N195F+V206Y+Y243F+E260K+W284D, W140Y+N195F+V206Y+Y243F+E260G+G476E, W140Y+W189G+N195F+V206Y+Y243F+E260G, W140Y+N195F+V206Y+Y243F+E260G+S303G, W140Y+W189T+N195F+V206Y+Y243F+E260G, W140Y+N195F+V206Y+Y243F+E260G+W284D, Y100I+W140Y+N195F+V206Y+Y243F+E260G, W140Y+N195F+V206Y+Y243F+E260G+G337N, W140Y+N195F+V206Y+Y243F+E260G+W439R, G109A+W140Y+E194D+N195F+V206Y+Y243F+E260G, G109A+W140Y+N195F+V206Y+Y243F+E260G+G476E, T51I+Y100I+G109A+W140Y+N195F+V206Y+Y243F+E260G, T51I+G109A+W140Y+N195F+V206Y+Y243F+E260G+W439R, T51I+S52Q+N54K+G109A+W140Y+N195F+V206Y+Y243F+E260G+G476E, W140Y+N195F+V206Y+Y243F+E260G+G304R+G476K, W140Y+N195F+V206Y+Y243F+E260G+W284R+G477K, W140Y+N195F+V206Y+Y243F+E260G+W284F+G477R, and N195F+V206Y+Y243F+E260G+W284D. 13. A composition according to claim 1 , wherein the variant further consists of substitutions at positions corresponding to the positions of the polypeptide of SEQ ID NO:1, selected from the group consisting of: W140Y+N195F+V206Y+Y243F+E260G+G477E, W140Y+N195F+V206Y+Y243F+E260T+W284D, G109A+W140Y+N195F+V206Y+Y243F+E260G, W140Y+N195F+V206Y+Y243F+E260G, N195F+V206Y+Y243F+E260K+W284D, W140Y+N195F+V206Y+Y243F+E260G+G476E, W140Y+W189G+N195F+V206Y+Y243F+E260G, W140Y+N195F+V206Y+Y243F+E260G+S303G, W140Y+W189T+N195F+V206Y+Y243F+E260G, W140Y+N195F+V206Y+Y243F+E260G+W284D, Y100I+W140Y+N195F+V206Y+Y243F+E260G, W140Y+N195F+V206Y+Y243F+E260G+G337N, W140Y+N195F+V206Y+Y243F+E260G+W439R, G109A+W140Y+E194D+N195F+V206Y+Y243F+E260G, G109A+W140Y+N195F+V206Y+Y243F+E260G+G476E, T51I+Y100I+G109A+W140Y+N195F+V206Y+Y243F+E260G, T51I+G109A+W140Y+N195F+V206Y+Y243F+E260G+W439R, T51I+S52Q+N54K+G109A+W140Y+N195F+V206Y+Y243F+E260G+G476E, W140Y+N195F+V206Y+Y243F+E260G+G304R+G476K, W140Y+N195F+V206Y+Y243F+E260G+W284R+G477K, W140Y+N195F+V206Y+Y243F+E260G+W284F+G477R, and N195F+V206Y+Y243F+E260G+W284D. 14. A composition according to claim 1 , wherein the variant further comprises alterations at positions corresponding to the positions of the polypeptide of SEQ ID NO:1, selected from the group consisting of: D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+G477E, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260T+W284D, D183″+G184*+G109A+W140Y+N195F+V206Y+Y243F+E260G, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+G476E, D183*+G184*+W140Y+W189G+N195F+V206Y+Y243F+E260G, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+S303G, D183*+G184*+W140Y+W189T+N195F+V206Y+Y243F+E260G, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+W284D, D183*+G184*+Y100I+W140Y+N195F+V206Y+Y243F+E260G, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+G337N, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+W439R, D183*+G184*+G109A+W140Y+E194D+N195F+V206Y+Y243F+E260G, D183*+G184*+G109A+W140Y+N195F+V206Y+Y243F+E260G+G476E, D183*+G184*+T51I+Y100I+G109A+W140Y+N195F+V206Y+Y243F+E260G, D183*+G184*+T51I+G109A+W140Y+N195F+V206Y+Y243F+E260G+W439R, D183*+G184*+T51I+S52Q+N54K+G109A+W140Y+N195F+V206Y+Y243F+E260G+G476E, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+G304R+G476K, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+W284R+G477K, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+W284F+G477R, and D183*+G184*+N195F+V206Y+Y243F+E260G+W284D. 15. A composition according to claim 1 , wherein the variant further consists of alterations at positions corresponding to the positions of the polypeptide of SEQ ID NO:1, selected from the group consisting of: D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+G477E, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260T+W284D, D183*+G184*+G109A+W140Y+N195F+V206Y+Y243F+E260G, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G, D183*+G184*+N195F+V206Y+Y243F+E260K+W284D, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+G476E, D183*+G184*+W140Y+W189G+N195F+V206Y+Y243F+E260G, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+S303G, D183*+G184*+W140Y+W189T+N195F+V206Y+Y243F+E260G, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+W284D, D183*+G184*+Y100I+W140Y+N195F+V206Y+Y243F+E260G, D183*+G184*+W140Y+N195F+V206Y+Y243F+E260G+G337N, D183″+G184″+W140Y+N195F+V206Y+Y243F+E260G+W439R, D183″+G184″+G109A+W140Y+E194D+N195F+V206Y+Y243F+E260G, D183″+G184″+G109A+W140Y+N195F+V206Y+Y243F+E260G+G476E, D183″+G184″+T51I+Y100I+G109A+W140Y+N195F+V206Y+Y243F+E260G, D183″+G184″+T51I+G109A+W140Y+N195F+V206Y+Y243F+E260G+W439R, D183″+G184″+T51I+S52Q+N54K+G109A+W140Y+N1

Assignees

Inventors

Classifications

C12Y302/01001
Alpha-amylase (3.2.1.1) · CPC title
C11D3/38681
Chemically modified or immobilised enzymes · CPC title
C12N9/2417Primary
from microbiological source · CPC title
C11D3/386Primary
Preparations containing enzymes {, e.g. protease or amylase} · CPC title

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What does patent US9284544B2 cover?: The present invention relates to cleaning compositions comprising variants of an alpha-amylase and methods of treating surfaces such as textiles with aqueous liquor comprising such compositions, especially at low temperatures.
Who is the assignee on this patent?: Jackson Michelle, Souter Philip Frank, Bewick Lindsay Suzanne, and 14 more
What technology area does this patent fall under?: Primary CPC classification C12N9/2417. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?: Publication date Tue Mar 15 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?: We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).