Promoter, promoter control elements, and combinations, and uses thereof

Patent metadata

Field	Value
Publication number	US-12331304-B2
Application number	US-202218067581-A
Country	US
Kind code	B2
Filing date	Dec 16, 2022
Priority date	Feb 1, 2008
Publication date	Jun 17, 2025
Grant date	Jun 17, 2025

Abstract

The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels in plants, and plants containing such promoters or promoter control elements.

First claim

What is claimed is:

1. A vector construct comprising:
a) a first nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:7, or a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO:7, wherein said first nucleic acid molecule functions as a promoter; and
b) a second nucleic acid molecule to be expressed,

wherein said first nucleic acid molecule and said second nucleic acid molecule are heterologous to each other and are operably linked.

2. The vector construct according to claim 1 , wherein said first nucleic acid molecule comprises the nucleic acid sequence of SEQ ID NO:7.

3. The vector construct according to claim 1 , wherein said second nucleic acid molecule encodes a polypeptide.

4. The vector construct according to claim 3 , wherein said second nucleic acid molecule is operably linked to said first nucleic acid molecule in the sense orientation.

5. The vector construct according to claim 4 , wherein said second nucleic acid molecule is transcribed into an RNA molecule that expresses the polypeptide encoded by said second nucleic acid molecule.

6. The vector construct according to claim 1 , wherein said second nucleic acid molecule is operably linked to said first nucleic acid molecule in the antisense orientation.

7. The vector construct according to claim 6 , wherein transcription of said second nucleic acid molecule produces an antisense molecule.

8. The vector construct according to claim 1 , wherein transcription of said second nucleic acid molecule produces an RNAi molecule against an endogenous gene.

9. The vector construct according to claim 3 , wherein said second nucleic acid molecule encodes a polypeptide of agronomic interest.

10. A plant or plant cell comprising the vector construct according to claim 1 .

11. A plant or plant cell stably transformed with the vector construct according to claim 1 .

12. A transgenic seed of the plant according to claim 10 .

13. A method of directing transcription comprising combining, in an environment suitable for transcription:
a) a first nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 7 or a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO:7, wherein said first nucleic acid molecule functions as a promoter;
and
b) a second nucleic acid molecule;
wherein said first nucleic acid molecule and said second nucleic acid molecule are heterologous to each other and operably linked, and transcribing said second nucleic acid molecule.

14. A method of expressing an exogenous coding region in a plant comprising:
(a) transforming a plant cell with the vector of claim 1 ;
(b) regenerating a stably transformed plant from the transformed plant cell of step (a); and
(c) selecting at least one plant containing the transformed plant cell,
wherein expression of the second nucleic acid molecule results in production of a polypeptide encoded by said second transcribable nucleic acid molecule.

15. A method of altering the expression of an endogenous gene in a plant comprising:
a) transforming a plant cell with the vector construct according to claim 1 , and
b) regenerating at least one stably transformed plant from said transformed plant cell, wherein transcription of said second nucleic acid molecule produces an RNAi molecule that suppresses expression of the endogenous gene.

16. A plant prepared according to the method of claim 14 .

17. A transgenic seed from the plant according to claim 16 .

18. A method of producing a transgenic plant, said method comprising:
(a) transforming a plant cell comprising the vector according to claim 1 ; and
(b) growing a plant from said plant cell.

19. The method of claim 18 , wherein said second nucleic acid molecule comprises a nucleic acid sequence encoding a polypeptide.

20. The method of claim 18 , wherein said second nucleic acid molecule is operably linked to said first nucleic acid molecule in an antisense orientation.

21. The method of claim 18 , wherein transcription of said second nucleic acid molecule produces an RNAi molecule.

22. A plant or plant cell comprising the vector construct according to claim 2 .

23. A plant or plant cell stably transformed with the vector construct according to claim 2 .

24. A transgenic seed of the plant according to claim 22 , wherein the transgenic seed comprises the vector construct.

25. A method of producing a transgenic plant, said method comprising:
(a) introducing into a plant cell the vector according to claim 2 ; and
(b) growing a plant from said plant cell.

Assignees

• Ceres Inc

Inventors

• Schneeberger Richard
• Margolles-Clark Emilio
• Tatarinova Tatiana
• Fang Yiwen
• Alexandrov Nickolai
• Medrano Leonard
• Pennell Roger
• Theiss Noah
• Grizard Danielle
• Davis Shauna J
• Robles Dennis
• Portereiko Michael

Classifications

• C12N15/8222 Primary

  Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation · CPC title

• C12N15/8234

  Seed-specific, e.g. embryo, endosperm · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title