Insulated serial column chromatography arrangements and systems

Patent metadata

Field	Value
Publication number	US-12320787-B2
Application number	US-202117354296-A
Country	US
Kind code	B2
Filing date	Jun 22, 2021
Priority date	Jun 22, 2020
Publication date	Jun 3, 2025
Grant date	Jun 3, 2025

Abstract

Exemplary embodiments eliminate the need for column ovens in serial column chromatography arrangements and systems by using insulated sleeves. The insulated sleeves may encase individual chromatography columns or clusters of chromatography columns. The use of the insulated sleeves allows the chromatography columns to be positioned in close proximity to each other. This may decrease the overall size of a serial column chromatography arrangement and may reduce costs by not requiring the column ovens.

First claim

The invention claimed is:

1. A chromatography columns arrangement, comprising:
a plurality of chromatography columns arranged in a longitudinally parallel cluster configuration;
a single insulating sleeve which collectively encompasses the chromatography columns in the cluster configuration and provides thermal management of the chromatography columns;
first and second end caps for the chromatography columns arrangements, the endcaps being sized to encompass the plurality of chromatography columns and to interface with the single insulating sleeve to form a sealed container;
an inlet valve for directing mobile phase with a sample of analyte into the cluster configuration, the inlet valve interfaces with the insulating sleeve and integrated into the first end cap for the arrangement;
an outlet valve for directing the mobile phase with the sample of analyte from a selected one of the chromatography columns to an output, the outlet valve interfaces with the insulating sleeve and integrated into the second end cap for the arrangement;
a controller configured for controlling the inlet valve and the outlet valve to serially pass the mobile phase with the sample of analyte through multiple successive ones of the plurality of a sequence of successive ones of the chromatography columns.

2. The chromatography columns arrangement of claim 1 , further comprising an inlet mobile phase heater for each of the chromatography columns in the configuration.

3. The chromatography columns arrangement of claim 1 , further comprising an outlet heater for each of the chromatography columns in the configuration.

4. The chromatography columns arrangement of claim 1 , wherein the inlet valve has an inlet port connected to an inlet tube for receiving the mobile phase with the sample of analyte and respective ports in fluid communication with a respective one of the chromatography columns.

5. The chromatography columns arrangement of claim 1 , wherein the outlet valve has an outlet port connected to an outlet tube for receiving the mobile phase with the sample of analyte and respective ports in fluid communication with a respective one of the chromatography columns.

6. The chromatography columns arrangement of claim 1 , further comprising a single inlet mobile phase heater for the arrangement.

7. The chromatography columns arrangement of claim 1 , further comprising fluidic connections from the outlet selection valve to inlets of at least some of the chromatography columns.

8. The chromatography columns arrangement of claim 1 , wherein a first of the chromatography columns in the plurality of chromatography columns is oriented to have an inlet fluidically coupled to the inlet selection valve and an outlet fluidically coupled to the outlet selection valve and a second of the chromatography columns in the plurality of chromatography columns is oriented to have an inlet fluidically coupled to the outlet selection valve and an outlet fluidically coupled to an inlet selection valve.

9. The chromatography columns arrangement of claim 1 , wherein the plurality of chromatography columns are liquid chromatography columns.

10. The chromatography columns arrangement of claim 1 , wherein the single insulating sleeves is a vacuum sleeve, a sleeve of insulating material or a sleeve containing gas.

11. The chromatography columns arrangement of claim 1 , wherein a first of the plurality of the chromatography columns has an inlet fluidically coupled to the inlet valve and a second of the plurality of the chromatography columns has an inlet fluidically coupled to the outlet valve.

12. The chromatography columns arrangement of claim 1 , wherein the controller is configurable to select a desired number of chromatography columns for the mobile phase with the sample of analyte to serially pass though.

13. The chromatography columns arrangement of claim 12 , wherein the desired number of chromatography columns is 2, 3, 4 or 5.

Assignees

• Waters Technologies Corp

Inventors

• Fogwill Michael O
• Gilar Martin
• Michienzi Joseph D
• Leveille Wade P
• Gerhardt Geoff C
• Musacchio Jeffrey
• Hill Jason F
• Cormier Sylvain G

Classifications

• G01N2030/3046

  temperature control of column inlet · CPC title

• G01N2030/326

  pumps · CPC title

• G01N2030/027

  Liquid chromatography · CPC title

• G01N2030/025

  Gas chromatography · CPC title

• G01N2030/328

  valves, e.g. check valves of pumps · CPC title