Techniques for thermally insulating a liquid chromatographic column

Patent metadata

Field	Value
Publication number	US-10413846-B2
Application number	US-201314406279-A
Country	US
Kind code	B2
Filing date	Jun 26, 2013
Priority date	Jul 6, 2012
Publication date	Sep 17, 2019
Grant date	Sep 17, 2019

Abstract

An apparatus for performing liquid chromatography includes a chromatography column, and an insulating member surrounding the chromatography column wherein the insulating member is formed from a vacuum chamber surrounding the chromatography column. Another apparatus for performing liquid chromatography includes a chromatography column, and an insulating member surrounding the chromatography column, wherein the insulating member includes aerogel. Also described is a method of insulating a chromatography column comprising forming a jacket surrounding the chromatography column, and creating a vacuum chamber in an area between the jacket and the chromatography column.

First claim

What is claimed is:

1. An apparatus for performing liquid chromatography comprising:
a liquid chromatography column;
a jacket surrounding an entirety of the liquid chromatography column; and
an insulating member surrounding the entirety of the liquid chromatography column, the insulating member comprising a vacuum chamber having the entirety of the liquid chromatography column arranged therein, the vacuum chamber formed in an area between the entirety of the liquid chromatography column and the jacket,
wherein the vacuum chamber comprises a gas, the gas comprising one of an inert gas or a heavy gas, wherein the inert gas or the heavy gas is at a pressure of about 10 −3 atm or less and a thermal conductivity of the inert or heavy gas within the vacuum chamber is about or less than 0.02 W/mK.

2. The apparatus of claim 1 , wherein the inert gas is any of argon, krypton, xenon, carbon dioxide and sulfur hexafluoride.

3. The apparatus of claim 1 , wherein the liquid chromatography column and the insulating member are integrated into a single component forming an insulated liquid chromatography column.

4. The apparatus of claim 1 , wherein the jacket is an outer layer of a housing of a column heater.

5. The apparatus of claim 1 , wherein the insulating member substantially prevents a radial thermal gradient from forming within the liquid chromatography column.

6. The apparatus of claim 1 , further comprising a heater that heats a mobile phase prior to an inlet of the liquid chromatography column.

7. The apparatus of claim 6 , wherein the heater and the liquid chromatography column are included in a column heater and the vacuum chamber is formed in an area between the liquid chromatography column and an outer wall of the column heater.

8. An apparatus for performing liquid chromatography, comprising:
a chromatography column; and
an insulating member surrounding the chromatography column wherein the insulating member is formed from a vacuum chamber surrounding the chromatography column, the vacuum chamber comprising a heavy gas,
wherein the heavy gas is at a pressure of about 10 −3 atm or less and a thermal conductivity of the heavy gas within the vacuum chamber is about or less than 0.02 W/mK.

9. The apparatus of claim 8 , the heavy gas comprising at least one of krypton, xenon, and sulfur hexafluoride.

10. The apparatus of claim 8 , the heavy gas comprising sulfur hexafluoride.

11. A liquid chromatography system, comprising:
a chromatography column; and
an insulating member comprising a vacuum chamber surrounding at least a portion of the chromatography column, the insulating member configured to cause a formation of a substantially adiabatic condition in the chromatography column during operation of the liquid chromatography system,
the vacuum chamber comprising a heavy gas, wherein the heavy gas is at a pressure of about 10 −3 atm or less and a thermal conductivity of the heavy gas within the vacuum chamber is about or less than 0.02 W/mK.

12. The liquid chromatography system of claim 11 , the substantially adiabatic condition formed without an application of heating to the chromatography column.

13. The liquid chromatography system of claim 11 , the substantially adiabatic condition formed without an application of cooling to the chromatography column.

14. The liquid chromatography system of claim 11 , the insulating member configured to substantially prevent a formation of a radial thermal gradient in the chromatography column during operation of the liquid chromatography system.

15. The liquid chromatography system of claim 14 , the insulating member configured to substantially prevent the formation of the radial thermal gradient without an application of heating or cooling to the chromatography column.

16. The liquid chromatography system of claim 11 , the insulating member configured to cause a formation of a substantially steady state temperature in the chromatography column during operation of the liquid chromatography system.

17. The liquid chromatography system of claim 16 , the insulating member configured to cause the formation of the substantially steady state temperature in the chromatography column without an application of heating or cooling to the chromatography column.

18. The liquid chromatography system of claim 16 , the steady state comprising a T in of a mobile phase entering the chromatography column, a T out of the mobile phase exiting the chromatography column, and an axial thermal gradient of the mobile phase being substantially constant.

19. The liquid chromatography system of claim 11 , the heavy gas comprising at least one of krypton, xenon, and sulfur hexafluoride.

20. The liquid chromatography system of claim 11 , the heavy gas comprising sulfur hexafluoride.

Assignees

• Waters Technologies Corp

Inventors

• Bouvier Edouard S P
• Iraneta Pamela C
• Luongo Joseph A
• Jarrell Joseph A

Classifications

• G01N2030/889

  monitoring the quality of the stationary phase; column performance · CPC title

• G01N2030/3084

  ovens · CPC title

• G01N30/88

  Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 · CPC title

• G01N30/54

  Temperature · CPC title

• G01N30/30

  of temperature · CPC title