Compound distribution in microfluidic devices

Patent metadata

Field	Value
Publication number	US-12298320-B2
Application number	US-202117538518-A
Country	US
Kind code	B2
Filing date	Nov 30, 2021
Priority date	Jun 27, 2019
Publication date	May 13, 2025
Grant date	May 13, 2025

Abstract

The present invention is related to the field of microfluidics and compound distribution within microfluidic devices and their associated systems. In one embodiment, present invention aims to solve the problem of molecule and compound absorbency into the materials making up laboratory equipment, microfluidic devices and their related infrastructure, without unduly restricting gas transport within microfluidic devices.

First claim

The invention claimed is:

1. A method of analyzing compound distribution in a system, comprising:
a) providing i) a first system comprising a first microfluidic device comprising a first membrane with pores in a plurality of regions, and ii) a first experimental protocol for use with said first system, said first experimental protocol comprising introducing a compound into said first microfluidic device and taking actions at one or more timepoints;
b) replacing said first system with a second system, said second system comprising a second microfluidic device comprising a second membrane without pores in at least one region in which said first membrane comprises pores;
c) modifying said first experimental protocol to generate a modified experimental protocol, wherein said modified experimental protocol comprises introducing said compound into said second microfluidic device and measuring compound concentration at one or more of said timepoints from said first experimental protocol;
d) performing said modified experimental protocol; and
e) using said measurement of concentration of said compound to analyze compound distribution across said system.

2. The method of claim 1 , further comprising the step of f) performing said first experimental protocol.

3. The method of claim 1 , wherein said system comprises infusion tubing.

4. The method of claim 1 , wherein said system comprises syringes.

5. The method of claim 1 , wherein said first experimental protocol comprises flowing fluid in said microfluidic device.

6. The method of claim 1 , wherein said first and second systems comprise first and second input ports configured to permit fluid input to said first and second microfluidic devices.

7. The method of claim 1 , wherein said first and second systems comprise first and second output ports configured to permit fluid output from said first and second microfluidic devices.

8. The method of claim 7 , wherein said first experimental protocol comprises collecting a first sample from said first output port.

9. The method of claim 1 , wherein said measuring of the concentration of said compound comprises collecting a sample from said second output port of said second microfluidic device and quantifying said concentration of said compound in said sample.

10. The method of claim 1 , wherein said modified experimental protocol further quantifies the percentage of said compound that is absorbed into said system.

11. The method of claim 2 , wherein said taking actions in said first experimental protocol comprises sampling effluent.

12. The method of claim 11 , wherein said first experimental protocol further comprises assaying said effluent to achieve an apparent metabolite value.

13. The method of claim 12 , further comprising using said measurement of concentration of said compound to correct said apparent metabolite value.

14. The method of claim 12 , further comprising using said measurement of concentration of said compound to determine variability of said apparent metabolite value.

15. The method of claim 1 , further comprising using said measurement of concentration to determine whether to perform said first experimental protocol.

16. The method of claim 1 , further comprising (i) using said measurement of concentration of said compound to generate a second modified experimental protocol; and (ii) performing said second modified experimental protocol.

17. The method of claim 1 , wherein said first experimental protocol comprises living cells.

Assignees

• Emulate Inc

Inventors

• Sliz Josiah
• Levner Daniel
• Zuckerman Brian
• Wen Norman
• Rubins Jonathan
• Shroff Tanvi
• Hinojosa Christopher David
• Ahn Grace
• Antontsev Victor
• Puerta Jefferson
• Conegliano David
• Kerns S Jordan

Classifications

• G01N2035/00544

  using fluid flow · CPC title

• G01N15/06

  Investigating concentration of particle suspensions (by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10) · CPC title

• B01L3/502707

  characterised by the manufacture of the container or its components · CPC title

• B01L2300/0681

  Filter · CPC title

• G01N2035/0097

  monitoring reactions as a function of time · CPC title